荔波樟江野生斑鳜线粒体Cyt b基因序列多态性分析  

Polymorphism Analysis of Mitochondria Cyt b Gene Sequences in Wild Population of LiBo Spot Mandarin Fish Siniperca scherzeri

作　　者：麻智芳 余科 安苗[1] 黄胜[1] 潘秋芝 李珊 何孝宽 MA Zhifang;YU Ke;AN Miao;HUANG Sheng;PAN Qiuzhi;LI Shan;HE Xiaokuan(College of Animal Science,Guizhou University,Guiyang 550025,China;Guizhou Vocational College of Agriculture,Guiyang 551499,China;Agriculture and Rural Affairs Bureau of Libo County,Qiannan 558400,China)

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]贵州农业职业学院,贵州贵阳551499 [3]荔波县农业农村局,贵州黔南558400

出　　处：《水产科学》2023年第1期21-29,共9页Fisheries Science

基　　金：国家自然科学基金资助项目(31660741);贵州省科技计划项目[(2017)5788]。

摘　　要：为探明贵州省荔波樟江野生斑鳜(简称荔波野生斑鳜)的遗传多样性水平和种质资源现状,科学保护和开发野生斑鳜种质资源,以线粒体DNA细胞色素b(Cyt b)基因作为分子标记,对野生斑鳜群体遗传多样性进行分析。研究结果显示,共获得75条长度为1141 bp的Cyt b基因同源序列,其碱基A、T、G和C的平均含量分别为24.4%、27.2%、15.5%和32.9%,A+T的含量(51.6%)略高于G+C(48.4%)的含量。共检测到17个变异位点,其中8个为简约信息位点,9个为单一信息位点。共定义15个单倍型,单倍型之间的平均遗传距离为0.003,邻接法构建的分子系统发育树显示,所有的单倍型均聚成一支;单倍型多样性指数和核苷酸多样性指数分别为0.613±0.064和0.00172±0.00021,平均核苷酸差异数为1.968。中性检验Tajima′s D值为-1.37917,差异不显著(P>0.10)。荔波漳江流域的野生斑鳜群体呈现高单倍型多样性和低核苷酸多样性,遗传多样性低,无种群扩张史,且与长江中上流的支流陆水河和洞庭湖水系的斑鳜群体有较高的亲缘性。研究结果可为当地斑鳜种质资源的保护和开发利用提供依据。In order to understand the status of germplasm resources and the level of genetic variation of wild population of LiBo spot mandarin fish Siniperca scherzeri, the cytochrome b(Cyt b) gene marker was selected for the genetic diversity analysis. The completed mt DNA Cytb gene sequence(1141 bp) of wild spot mandarin fish in Zhangjiang Libo County, Guizhou Province, was obtained by PCR amplification and sequence analysis. The amplification showed that the average contents of A,T,G and C were 24.4%, 27.2%, 15.5% and 32.9%, respectively, a little higher average content of A+T(51.6%) than that of G+C(48.4%). Seventeen variation sites were detected, in these variation sites where eight were parsimony informative sites and nine were singleton variable sites. Fifteen haplotypes were identified in 75 individuals of the population, with the average genetic distance of 0.003 among Cyt b haplotypes, and the phylogenetic tree revealed that fifteen Cyt b haplotypes were clustered into one clade, with haplotype diversity of 0.613±0.064 and nucleotide diversity of 0.00172±0.00021, and the average number of nucleotide differences being 1.986. The Tajima′s D value of neutrality test was-1.37917, without significant difference(P>0.10). These findings indicated that the wild population of LiBo spot mandarin fish had low genetic diversity, as high haplotype diversity and low nucleotide diversity. The population demographic analyses revealed that the wild population of LiBo spot mandarin fish had a stable population without experiencing population expansion, with the closest evolutionary relationship with the spot mandarin fish from Yangtze River(Lushui and Dongting)according to a phylogenetic study. The finding provides reference for the protection and utilization of its germplasm resources.

关 键 词：斑鳜 细胞色素b基因(Cyt b) 樟江 遗传多样性 

分 类 号：Q786[生物学—分子生物学]