野生和养殖丁[鱼岁]肠道微生物菌群的比较分析  

Comparative Analysis on Intestinal Microflora of Wild and Cultured Tench Tinca tinca

作　　者：钱逸 许沁宇 钱龙[2] 艾涛[2] 向伟[2] 胡伯林[2] 王军[1] 王成辉[1] QIAN Yi;XU Qinyu;QIAN Long;AI Tao;XIANG Wei;HU Bolin;WANG Jun;WANG Chenghui(Shanghai Engineering Research Center of Aquaculture,National Demonstration Centre for Experimental Fisheries Science Education,Key Laboratory of Freshwater Aquatic Genetic Resources Certificated by Ministry of Agriculture and Rural Affairs,Shanghai Ocean University,Shanghai 201306,China;Fisheries Technology Extension Station,Xinjiang Production and Construction Corps,rümqi 830002,China)

机构地区：[1]上海海洋大学,农业农村部淡水水产种质资源重点实验室,水产科学国家级实验教学示范中心,上海水产养殖工程技术研究中心,上海201306 [2]新疆生产建设兵团水产技术推广总站,新疆乌鲁木齐830002

出　　处：《水产科学》2023年第1期128-135,共8页Fisheries Science

基　　金：新疆生产建设兵团科学技术局项目(2016AC027)。

摘　　要：鱼类肠道菌群的结构和功能对其生长发育起重要作用。丁[鱼岁]是我国新疆地区特有的经济养殖鱼类,为评估野生丁[鱼岁]和养殖丁[鱼岁]肠道微生物菌群的结构和差异,以野生丁[鱼岁]和养殖丁[鱼岁]作为研究对象,采用Illumina Miseq高通量测序方法对其肠道16S rRNA基因的V3~V4区进行测序,比较两种环境条件下丁[鱼岁]肠道微生物菌群结构的丰度变化和差异。试验结果显示,丁[鱼岁]肠道微生物的优势菌群主要为变形菌门、厚壁菌门、放线菌门和拟杆菌门。但在两种环境下,其肠道菌群结构存在明显差异:在门水平下,梭菌门和酸菌门在养殖丁[鱼岁]中的丰度显著低于野生丁[鱼岁](P<0.05);在属水平下,乳酸杆菌属和不动杆菌属在养殖丁[鱼岁]中的丰度高于野生丁[鱼岁],而支原体和黄杆菌属则显著低于野生丁[鱼岁](P<0.01);在种水平下,洛菲不动杆菌在养殖丁[鱼岁]中的含量显著高于野生丁[鱼岁](P<0.01),而未分类的黄杆菌则显著低于野生丁[鱼岁](P<0.05)。试验结果表明,由于饲养环境的改变,野生丁[鱼岁]和养殖丁[鱼岁]肠道菌群的组成及丰度出现显著变化。该结果可为后续丁[鱼岁]肠道微生物的研究和养殖管理提供基础数据和参考。The bacterial community and function of the intestinal microbiota play important roles in the growth and development of fish. Tench Tinca tinca is a cultured fish species with high economic value in Xinjiang area, China. In order to compare the structure and abundance of intestinal microbial communities between the wild and cultured tench, the 16S rRNA gene(V3—V4 region) was sequenced between wild and cultured tench using Illumina Miseq high-throughput sequencing method. The results showed that the dominant intestinal microbial flora in tench was mainly Proteobacteria, Firmicutes, Actinobacteriota, and Bacteroidota. However, significant intestinal microbiota was identified between wild and cultured tench. In detail, at phylum level, the abundance of Fusobacteriota and Acidobacteriota in the cultured tench was significantly lower than that in the wild tench(P<0.05). At genus level, the abundance of Lactobacillus, and Acinetobacter was higher in the cultured tench than that in the wild tench, and the abundance of Mycoplasmataceae and Flavobacterium were lower in the cultured tench(P<0.01). At species level, the abundance of A. lwoffii in the cultured tench was higher than that in the wild tench(P<0.01), while the unclassified Flavobacterium was significantly lower in the wild tench(P<0.05). It was found that the community and abundance of the intestinal microbiome was significantly different between wild and cultured tench. Our findings provide basic data for the research of intestinal microbiome and cultivation management of tench in future.

关 键 词：丁[鱼岁] 肠道微生物 环境 

分 类 号：S917.1[农业科学—水产科学]