Chemical constituents and theiranti-infective activity of Paeonia suffruticosa  

作　　者：Yang Wu Si-Yun Liu Chun-Hua Lu Yue-Mao Shen 

机构地区：[1]Key Laboratory of Chemical Biology(Ministry of Education),School of Pharmaceutical Sciences,Cheeloo College of Medicine,Shandong University,Jinan,Shandong 250012,China [2]Department of Materials and Chemical Engineering,Chizhou University,Anhui,247000,China

出　　处：《TMR Integrative Medicine》2022年第25期1-5,共5页TMR整合医学

基　　金：National Natural Science Foundation of China(2217070336,81673317);the Program for Changjiang Scholars andInnovative Research Team in University(IRT_17R68)。

摘　　要：Objective:Research the chemical constituents of Paeonia suffruticosa that are responsible for its anti-infective properties.Methods:Several column chromatographic methods were used to purify the chemical constituents from P.suffruticosa,including medium pressure liquid chromatography,Sephadex LH-20,and normal silica gel.A disc diffusion method was used to screen for antibacterial activity,and their anti-virulence activity was assessed on the type III secretion system(T3SS)of Salmonella pathogenicity island 1(SPI-1)in Salmonella enterica serovar Typhimurium UK-1 g 8956 by SDS-PAGE and western blots.Results:Twenty-one compounds were identified.Compounds 7,8 and 17 showed moderate activity against S.aureus ATCC25923,compounds 8,9 and 10 showed weak activities against B.subtilis ACCC11060.Meanwhile,phenols(14-18)and flavonoids(20 and 21)inhibited T3SS protein secretion of S.typhimurium without affecting bacterial growth.Furthermore,a strong inhibitory effect was observed for 17 and 20 on SPI-1 mediated invasion of HeLa cells.Additionally,no toxicity was observed for these compounds.Conclusion:P.suffruticosa has anti-infective properties due in part to the fact that phenols and flavonoids can block the secretion of T3SS-associated protein effectors.

关 键 词：Paeonia suffruticosa anti-infective activity type III secretion system phenols and flavonoids 

分 类 号：R284.1[医药卫生—中药学]