Effects of N-acetylcysteine on growth,viability and reactive oxygen species levels in small antral follicles cultured in vitro  

作　　者：Efigênia B.Cordeiro Bianca R.Silva Laís R.F.M.Paulino Pedro A.A.Barroso Laryssa G.Barrozo Miguel F.de Lima Neto JoséR.V.Silva 

机构地区：[1]Laboratory of Biotechnology and Physiology of Reproduction,Federal University of Ceara,Av.Comandante Maurocélio Rocha Ponte 100,62041-040,Sobral,CE,Brazil

出　　处：《Asian pacific Journal of Reproduction》2023年第1期42-48,共7页亚太生殖杂志（英文版）

基　　金：supported by grants from the National Council for Scientific and Technological Development(CNPq,Brazil,grant number 308737/2018-0).

摘　　要：Objective:To investigate the effects of different concentrations of N-acetylcysteine on follicular growth and morphology,as well as on viability,levels of reactive oxygen species(ROS)and meiotic progression of oocytes from in vitro cultured bovine early antral follicles.Methods:Isolated early antral follicles(about 500μm)were cultured in TCM-199+alone or supplemented with 1.0,5.0 or 25.0 mM N-acetylcysteine at 38.5℃with 5%CO_(2) for 8 days.Follicle diameters were evaluated at day 0,4 and 8 of culture.At the end of culture,the levels of ROS,chromatin configuration and viability(calcein-AM and ethidium homodimer-1 staining)were investigated in the cumulus-oocyte complexes.Comparisons of follicle diameters between treatments were performed.Data on percentages of morphologically normal follicles,growth rates and chromatin configuration in different treatments were compared.Results:An increase in follicular diameters after culture in all treatments was observed,except for follicles cultured with 25.0 mM N-acetylcysteine.Fluorescence microscopy showed that oocytes cultured in all treatments were stained positively with calcein AM,and that 5.0 mM N-acetylcysteine reduced fluorescence for ethidium homodimer-1.Intracellular levels of ROS in oocytes from follicles cultured with 1.0 mM N-acetylcysteine showed a significant reduction compared to other treatments.The presence of N-acetylcysteine in culture medium did not influence the rates of oocyte at the germinal vesicle stage.Conclusions:N-acetylcysteine at concentrations of 1.0 and 5.0 mM reduces ROS levels and staining for ethidium homodimer-1 in in vitro cultured follicles,respectively,while 25.0 mM N-acetylcysteine decreases follicular growth and the percentages of continuously growing follicles.

关 键 词：ANTIOXIDANT N-ACETYLCYSTEINE Antral follicles BOVINE Reactive oxygen species 

分 类 号：R714.8[医药卫生—妇产科学]