复杂电磁环境工作人员尿中8-羟基脱氧鸟苷和8-羟基鸟苷水平及其影响因素分析  

Urinary 8-hydroxydeoxyguanosine and 8-hydroxyguanosine levels and their influencing factors on workers under complex electromagnetic environment

作　　者：闵之藤 石梦蝶[2] 殷文军 梁娇君 陈振龙 易桂林 MIN Zhi-teng;SHI Meng-die;YIN Wen-jun;LIANG Jiao-jun;CHEN Zhen-long;YI Gui-lin(Wuhan Prevention and Treatment Center for Occupational Diseases,Wuhan,Hubei 430015,China;不详)

机构地区：[1]武汉市职业病防治院职业卫生科,湖北武汉430015 [2]武汉市疾病预防控制中心环境健康与食品安全所

出　　处：《现代预防医学》2023年第1期76-79,96,共5页Modern Preventive Medicine

基　　金：武汉科技大学职业危害识别与控制湖北省重点实验室项目(OHIC2018Y04);武汉科技大学职业危害识别与控制湖北省重点实验室项目(OHIC2021Y12)。

摘　　要：目的 对复杂电磁环境(complex electromagnetic environment, CEE)工作人员的DNA和RNA氧化损伤情况以及其影响因素进行分析,为预防和控制职业病危害提供科学依据。方法 采用方便抽样法选择武汉某芯片厂CEE工作人员(n=133)为暴露组和办公室人员(n=56)为对照组,用稀释法和液相色谱-三重四级杆质谱仪测量氧化应激标志物8-羟基脱氧鸟苷(8-OHdG)和8-羟基鸟苷(8-OHG)在其尿液样本中的浓度。根据问卷调查结果,用logistic回归法分析影响8-OHdG的相关因素。结果 暴露组人员尿中的8-OHdG和8-OHG,经尿比重调整后浓度中位数分别为9.34 ng/ml和17.08 ng/ml。与对照组相比,暴露组8-OHdG浓度高于对照组,且差异有统计学意义(Z=-2.194,P=0.028)。CEE(OR=3.07,95%CI:1.35~7.00)、吸烟(OR=2.62,95%CI:1.13~6.08)和运动(OR=2.5,95%CI:1.04~6.00)为危险因素;女性(OR=0.09,95%CI:0.03~0.28)为保护因素。结论 长期暴露于复杂电磁环境(CEE)可引起氧化应激损伤,应加强此类职业人群的劳动保护。Objective To provide a scientific basis for the prevention and control of occupational hazard by studying the oxidative damage of DNA/RNA in complex electromagnetic environment(CEE) workers and its influencing factors. Methods The concentrations of oxidative stress markers 8-hydroxydeoxyguanosine(8-OHdG) and 8-hydroxyguanosine(8-OHG) in urine samples were analyzed by dilution method and liquid chromatography-triple quadrupole mass spectrometry(LC-MS). CEE workers(n =133) in a chip factory in Wuhan were selected as the exposure group and office workers(n=56) as the control group. According to the survey results, the influencing factors of 8-OHdG were analyzed by multivariate logistic regression. Results The median concentrations of 8-OHdG and 8-OHG in urine of the exposed group were 9.34 ng/ml and 17.08 ng/ml respectively. Compared with the control group, the concentration of 8-OHdG in exposed group was higher than that in the control group, and the difference was statistically significant(Z=-2.194, P=0.028). CEE(OR=3.07, 95%CI: 1.35-7.00), smoking(OR=2.62, 95%CI: 1.13-6.08) and exercise(OR=2.5, 95%CI: 1.04-6.00) were risk factors. Female(OR=0.09, 95%CI: 0.03-0.28) was a protective factor. Conclusion Long-term exposure to CEE can cause oxidative stress injury, so labor protection should be strengthened for this kind of occupational population.

关 键 词：复杂电磁环境 8-羟基脱氧鸟苷 8-羟基鸟苷 影响因素 

分 类 号：R135[医药卫生—劳动卫生]