唐山市新诊断未治疗HIV-1感染者中HIV遗传多样性及分子传播网络分析  

HIV-1 genetic diversity and molecular transmission network among newly diagnosed HIV-1 individuals with naive-treatment,China

作　　者：孙百虎[1] 张庆娟[2] 唐湘云 路新利 SUN Bai-hu;ZHANG Qing-juan;TANG Xiang-yun;LU Xin-li(Shijiazhuang University of Applied Technology,Shijiazhuang,Hebei 050081,China;不详)

机构地区：[1]石家庄职业技术学院,河北石家庄050081 [2]唐山市疾病预防控制中心 [3]华北理工大学公共卫生学院 [4]河北省疾病预防控制中心艾滋病所

出　　处：《现代预防医学》2023年第1期159-162,192,共5页Modern Preventive Medicine

基　　金：河北省医学科学研究重点课题计划(20160061);河北省自然科学基金(H2016303006)。

摘　　要：目的了解未治疗人群中HIV-1遗传基因多样性和分子传播网络。方法使用in-house的方法进行HIV-1耐药基因型检测,将获得的199条唐山市HIV-1 pol序列上传至斯坦福大学耐药数据库进行耐药分析;使用Cytoscape_v3.2.0软件对遗传基因距离≤0.015的序列构建分子传播网络。结果在获得的199条序列中共有8种基因亚型被确认,其中CRF01_AE流行率最高,占到43.7%(93/199),其次是CRF07_BC(30.7%,61/199)、URFs(10.1%,20/199)、B(8.5%,17/199)、CRF55_01B(2.5%,5/199)、CRF65_cpx(0.5%,1/199)、CRF59_01B(0.5%,1/199)和CRF08_BC(0.5%,1/199)。传递性耐药(TDR)基因突变发生率为4.5%(9/199),其中非核苷类逆转录酶抑制剂(NNRTIs)、蛋白酶抑制剂(PIs)和核苷类逆转录酶抑制剂(NRTIs)的TDR基因突变发生率依次为2.0%(4/199)、2.0%(4/199)和0.5%(1/199)。共有25条入网的序列产生了5个分子传播网络,CRF07_BC簇1是最大传播簇。结论整体TDR耐药率处于低水平流行,但HIV-1在北京与河北之间经性传播风险较高,应重点加强此类人群的监测,提示了在北京与唐山市HIV-1感染者之间通过性接触存在紧密的传播关系。Objective To investigate the epidemic of HIV-1 genetic diversity and molecular transmission network.Methods HIV-1 drug resistance genotype assay was carried out using in-house methods,and our study sequences were submitted to Stanford Drug Resistance Database for drug resistance analysis.Molecular transmission networks were visualized by using Cytoscape_v3.2.0 with a threshold genetic distance of 0.015.Results Among 199 sequences successfully obtained,eight HIV-1 subtypes were confirmed in this study.Among them,CRF01_AE was the most frequent,accounting for 43.7%(93/199),followed by CRF07_BC(30.7%,61/199),URFs(10.1%,20/199),B(8.5%,17/199),CRF55_01B(2.5%,5/199),CRF65_cpx(0.5%,1/199),CRF59_01B(0.5%,1/199)and CRF08_BC(0.5%,1/199).The overall TDR rate was 4.5%(9/199),corresponding to 2.0%(4/199)for non-nucleoside reverse transcriptase inhibitors(NNRTIs),2.0%(4/199)for protease inhibitors(PIs)and 0.5%(1/199)for nucleoside reverse transcriptase inhibitors(NRTIs).Five molecular transmission networks were constructed using 25 sequences.Among five,CRF07_BC cluster 1 was the biggest transmission network.Conclusion The overall TDR rate is low,while HIV-1 is spread at higher risk in the sexual contact population between Beijing and Hebei,suggesting the surveillance of TDR in this population should be strengthened and there are the close transmission relationship between Beijing and Hebei via sexual contact.

关 键 词：人免疫缺陷病毒 传递性耐药 基因亚型 分子传播网络 

分 类 号：R512.91[医药卫生—内科学]