6%羟乙基淀粉130/0.4对创伤性家兔炎症介质及其信号通路的影响  

Effects of 6% hydroxyethyl starch 130/0.4 on inflammatory mediators and signal pathways in traumatic rabbits

作　　者：吕洪锦 张倩 黄绍艳[3] 张建中[3] LV Hongjin;ZHANG Qian;HUANG Shaoyan;ZHANG Jianzhong(Department of Anesthesiology,Longkou People Hospital,Yantai,Shandong 265700,China;Second Clinical Medical College,Binzhou Medical university,Yantai,Shandong 264000,China;Department of Anesthesiology,Yantaishan Hospital,Yantai,Shandong 264000,China)

机构地区：[1]龙口市人民医院麻醉科,山东烟台265700 [2]滨州医学院第二临床医学院,山东烟台264000 [3]烟台市烟台山医院麻醉科,山东烟台264000

出　　处：《现代医药卫生》2023年第2期211-215,219,共6页Journal of Modern Medicine & Health

基　　金：山东省自然科学基金面上项目(ZR2022MH320);山东省烟台市科技创新发展计划项目(2021MSGY048);滨州医学院“临床+X”科技创新项目(BY2021LCX35)。

摘　　要：目的 探讨6%羟乙基淀粉(HES)130/0.4对创伤性家兔炎症介质及其信号通路——微小RNA(miR)-146a/Toll样受体-4(TLR4)/核因子-κB(NF-κB)的影响。方法 2022年4月选取新西兰雄性家兔16只,麻醉后用钝器敲打家兔的后肢造成后肢骨折,然后行骨折切开内固定术。常规备皮、消毒,骨折部位外侧切口,显露股骨骨折部位,胯骨折线置入合适的国产四孔钢板,在骨折对应的肌肉组织2 cm×2 cm制作软组织缺损模型,同时,取血液和骨折部分肌肉组织备检(T_(0))。将家兔采用随机数字表法分为乳酸钠林格(LR)组和HES组,每组8只。LR组注射10 mL/kg LR,HES组注射10 mL/kg HES,每天1次,输注5 d,结束后将家兔麻醉取损伤部位肌肉组织备检(T_(1))。采用酶联免疫吸附试验法测定T_(0)、T_(1)时家兔血液肿瘤坏死因子-α(TNF-α)、白细胞介素-1(IL-1)、IL-6等,蛋白质印迹法测定T_(0)、T_(1)时家兔肌肉组织NF-κB P65、TLR4、IL-1受体相关激酶1(IRAK1)和TNF受体相关因子6(TRAF6)蛋白,逆转录-聚合酶链反应法检测T_(0)、T_(1)时家兔肌肉组织miR-146a。结果 与T_(0)时比较,2组家兔T_(1)时血液TNF-α、IL-1、IL-6水平,以及肌肉miR-146a、NF-κB P65、TLR4、IRAK1、TRAF6蛋白水平均明显升高,差异均有统计学意义(P<0.05)。与LR组比较,HES组家兔T_(1)时血液TNF-α、IL-1、IL-6水平,以及肌肉组织NF-κB P65、TLR4、IRAK1、TRAF6蛋白水平均明显降低,肌肉组织miR-146a表达水平明显升高,差异均有统计学意义(P<0.05)。结论 HES可能通过上调miR-146a表达水平,负反馈调节TLR4信号通路上的IRAK1、TRAF6,抑制炎症介质的产生而减轻创伤家兔的炎性反应。Objective To investigate the effect of 6% hydroxyethyl starch(HES) 130/0.4 on the inflammatory mediators and its signal pathway micro RNA(miR)-146a/Toll-like receptor 4(TLR4)/nuclear factor-κB(NF-κB) in traumatic rabbits.Methods A total of 16 New Zealand male rabbits were selected in April 2022.After anesthesia, the hind limbs of the rabbits were beaten with blunt objects to cause hind limb fractures, and then the fractures were treated with open internal fixation.The skin was prepared and disinfected, the lateral incision of the fracture site was exposed, and the femur fracture site was exposed, after that a suitable domestic four-hole steel plate was placed across the fracture line.A soft tissue defect model was made in the muscle tissue corresponding to the fracture of about 2 cm×2 cm, and at the same time, the muscle tissue of the blood fracture was taken for backup examination(T_(0)).According to random number table method, the rabbits were divided into the sodium lactate Ringer(LR) group and the HES group, with eight rabbits in each group.The LR group was injected with 10 mL/kg LR,while the HES group was injected 10 mL/kg HES once a day for five days.After the infusion rabbits were anesthetized, the muscle tissue of the injured site was taken for backup examination(T_(1)).The enzyme-linked immunosorbent assay(ELISA) was used to determine tumor necrosis factor-α(TNF-α),interleukin-1(IL-1) and IL-6 in blood of rabbits at T_(0)and T_(1),and western blotting was used to determine NF-κB P65,TLR4,IL-1 receptor-associated kinase 1(IRAK1),and TNF receptor-associated factor 6(TRAF6) protein levels in the muscle tissue at T_(0)and T_(1).The reverse transcription-polymerase chain reaction was used to determine miR-146a in the rabbit muscle tissue at T_(0)and T_(1).Results Compared with T_(0),the levels of TNF-α,IL-1,IL-6 in blood, and the levels of miR-146a, NF-κB P65,TLR4,IRAK1,and TRAF6 protein in muscle of the two groups were significantly higher at T_(1),and the differences were statistically significant(P

关 键 词：羟乙基淀粉 微小RNA-146a TOLL样受体4 NF-κB 兔 炎性反应 

分 类 号：R641[医药卫生—外科学]