基于TOPSIS法对嫁接提高黄瓜耐Ca(NO_(3))_(2)胁迫的综合评价  

Comprehensive evaluation of grafting improving cucumber’s tolerance to Ca(NO_(3))_(2) stress based on TOPSIS

作　　者：郭佩金 陈小玲 王智钰 梁佳毅 王德亨 甄爱[1,2,3] GUO Peijin;CHEN Xiaoling;WANGZhiyu;LIANG Jiayi;WANG Deheng;ZHEN Ai(School of Horticulture,Northwest A&F University,Yangling Shaanxi 712100,China;Key Laboratory of Protected Horticultural Engineering in Northwest,Ministry of Agriculture and Rural Affairs,Yangling Shaanxi 712100,China;Shaanxi Province Facility Agriculture Engineering Center,Yangling Shaanxi 712100,China)

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100 [2]农业农村部西北设施园艺工程重点实验室,陕西杨凌712100 [3]陕西省设施农业工程中心,陕西杨凌712100

出　　处：《东北农业大学学报》2023年第1期33-44,共12页Journal of Northeast Agricultural University

基　　金：陕西省自然科学基金项目(2021JQ-142)。

摘　　要：为探究砧木嫁接提高黄瓜耐盐性调控机制,获得嫁接黄瓜耐盐性综合评价方法,以盐敏感型黄瓜(Cucumis sativus L.)‘津春4号’自嫁苗及其与南瓜(Cucurbita moschata Duch.)‘强力士’和‘青藤台木’的嫁接苗为试验材料,研究Ca(NO_(3))_(2)胁迫对不同砧木嫁接黄瓜幼苗生长、根系形态、光合荧光特性、抗氧化能力和渗透调节能力的影响,采用多指标协同评判法评价植株耐盐性。结果表明,Ca(NO_(3))_(2)胁迫下黄瓜自嫁苗植株中丙二醛(MDA)含量显著增加,植株生长和生理受到严重抑制,砧木嫁接可通过增加超氧化物歧化酶活性(SOD)、过氧化物酶活性(POD)活性、脯氨酸和可溶性糖含量提高黄瓜幼苗抗氧化能力和渗透调节能力,减轻Ca(NO_(3))_(2)胁迫对植株根系和叶片净光合速率(Pn)、气孔导度(Gs)、非光化学淬灭系数(NPQ)和PSII有效光化学量子效率(Fv’/Fm’)等光合荧光特性的破坏,缓解Ca(NO_(3))_(2)胁迫对黄瓜幼苗生长的不利影响。植株耐盐性综合评价结果表明南瓜品种‘强力士’是适于提高黄瓜耐盐性的优良砧木。In order to explore the regulatory mechanism of rootstock grafting to improve the salt tolerance of cucumber,and to obtain a comprehensive evaluation method for the salt tolerance of grafted cucumber,a salt-sensitive cucumber(Cucumis sativus L.)cultivar,’Jinchun No.4’was used as scion and two salt-tolerant pumpkin cultivar,’Qianglishi’and’Qingtengtaimu’(Cucurbita moschata Duch.)were selected as rootstock.The effects of Ca(NO_(3))_(2)stress on the plant growth,root morphology,photosynthetic fluorescence characteristics,antioxidant capacity and osmotic adjustment capacity of cucumber seedlings grafted on different rootstocks were studied,and the salt tolerance of the plant was evaluated by multi-index collaborative evaluation method.The results showed that under Ca(NO_(3))_(2)stress,MDA content in cucumber seedlings significantly increased,and plant growth and physiology were severely inhibited.However,rootstock grafting could improve the antioxidant capacities and osmotic adjustment capacities of cucumber seedlings by increasing the activities of SOD and POD,proline and soluble sugar contents,thereby reducing the damage of Ca(NO_(3))_(2)stress on the plant roots and photosynthetic fluorescence characteristics in leaves,such as Pn,Gs,NPQ and Fv’/Fm’.Finally,the adverse effects of Ca(NO_(3))_(2)stress on the growth of cucumber seedlings were alleviated.The comprehensive evaluation of plant salt tolerance showed that the pumpkin variety’Qianglishi’was an excellent rootstock suitable for improving the salt tolerance of cucumber.

关 键 词：黄瓜 砧木嫁接 Ca(NO_(3))_(2)胁迫 耐盐性 

分 类 号：S642.2[农业科学—蔬菜学]