猪嵌合RNA BCL2L2-PABPN1剪接调控、表达与亚细胞定位分析  

Splicing regulation,expression and subcellular localization of chimeric RNA BCL2L2-PABPN1 in pigs

作　　者：杨秀芹[1] 张倩 李佳欣 郝婉君 张晓涵 何鑫淼[2] 庞宇[1] YANG Xiuqin;ZHANG Qian;LI Jiaxin;HAO Wanjun;ZHANG Xiaohan;HE Xinmiao;PANG Yu(School of Animal Sciences and Technology,Northeast Agricultural University,Harbin 150030,China;Institute of Animal Husbandry,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China)

机构地区：[1]东北农业大学动物科学技术学院,哈尔滨150030 [2]黑龙江省农业科学院畜牧研究所,哈尔滨150086

出　　处：《东北农业大学学报》2023年第1期79-87,共9页Journal of Northeast Agricultural University

基　　金：黑龙江省科研业务费(CZKYF2020A004);黑龙江省博后基金项目(LBH-Z20041);中国博士后科学基金项目(2020M670876)。

摘　　要：相邻基因间顺式剪接(cis-splicing of adjacent gene,cis-SAGe)产生的嵌合RNA在细胞生长、疾病发生等生理活动中发挥重要作用,为探究cis-SAGe剪接调控机制,研究在前期克隆BCL2L2-PABPN1(BP)基础上,利用minigene、定点突变、瞬时转染、半定量RT-PCR以及生物信息学分析等方法分析BP转录后剪接的调控元件,发现thyroid hormone receptor exon 9、gh-1 intron 3、srp40-exonic splicing enhancer、β-tropomyosin exon 6b等剪接增强子元件在嵌合子形成中发挥重要作用。同时比较分析BP与两个亲本基因组织表达以及亚细胞定位情况。研究结果将为进一步揭示BP转录后调控机制及功能提供基础。Chimeric RNA produced by cis-splicing of adjacent genes(cis-SAGe)plays an important role in cell growth,disease occurrence and other physiological activities.To explore the regulatory mechanism of cis-SAGe splicing.In this study,on the basis of previous cloning of BCL2L2-PABPN1(BP),minigene,site-directed mutagenesis,transient transfection,semi-quantitative RT-PCR and bioinformatics analysis were used to analyze the regulatory elements of BP splicing after transcription.It was found that splicing enhancer elements such as thyroid hormone receptor exon 9,gh-1 intron 3,srp 40-exonic splicing enhancer,andβ-tropomyosin exon 6b played important roles in chimeric cell formation.At the same time,the tissue expression and subcellular localization of BP and the two parent genes were compared and analyzed.The results would provide a basis for further revealing the post-transcriptional regulatory mechanism and function of BP.

关 键 词：猪 嵌合RNA BCL2L2-PABPN1 剪接 顺式调控元件 

分 类 号：Q953[生物学—动物学]