紫外分光光度法检测海南霉素的研究  

Research on the Detection of Hainanmycin by UV Spectrophotometry

作　　者：池可心 姚卫蓉[1] CHI Ke-xin;YAO Wei-rong(School of Food Science and Technology,Jiangnan University,Wuxi,Jiangsu 214122,China)

机构地区：[1]江南大学食品学院,江苏无锡214122

出　　处：《粮油食品科技》2023年第1期173-180,共8页Science and Technology of Cereals,Oils and Foods

基　　金：国家重点研发计划(2018YFC1602300)。

摘　　要：海南霉素钠(C_(47)H_(79)O_(15)Na)是一种广泛添加在鸡、牛羊等动物饲料中的新型一元酸聚醚类抗生素,主要用于防治球虫病、调节生长代谢。利用海南霉素与衍生剂发生显色反应,可以通过紫外分光光度法进行检测。结果表明,对二甲氨基苯甲醛、香草醛两种衍生剂对海南霉素衍生后衍生产物分别在595 nm、590 nm处有最大紫外吸收,海南霉素经香草醛衍生后检测限最低,当衍生剂组成为4%香草醛(g/100 mL)、20%盐酸和80%无水乙醇时,与海南霉素溶液按照4∶6(V∶V)的比例在90℃下衍生化反应5 min,衍生后显色效果最好,海南霉素在5~50μg/mL浓度范围内与吸光度值呈线性关系,决定系数R^(2)为0.993,可用于定量检测预混剂和饲料中的海南霉素含量。在饲料中定量限为4.8 mg/kg,在饲料、预混剂中海南霉素回收率分别为75%~94%、83%~101%,可作为饲料和海南霉素钠预混剂中检测海南霉素的一种有效方法。Hainanmycin sodium is a new type of monobasic acid polyether antibiotics,which has been widely used in chicken,cattle and sheep and other animal feeds,and it is mainly used to prevent coccidiosis and regulate growth metabolism.The color reaction between hainanmycin and derivative can be detected by UV spectrophotometry.The results showed that the derivatives of p-dimethylaminobenzaldehyde and vanillin had the maximum UV absorption at 595 nm and 590 nm,respectively,and hainanmycin had the lowest detection limit after vanillin derivatization.When the derivative agent was composed of 4%vanillin(g/100 mL),20%hydrochloric acid and 80%anhydrous ethanol,the derivative reaction with hainanmycin solution was carried out at 90℃for 5 min at the volume ratio of 4∶6(V∶V),and the color effect was the best after derivatization.Hainanmycin showed a linear relationship with the absorbance in the concentration range of 5~50μg/mL,and the R^(2)(Coefficient of determination)was 0.993.This method could quantify the hainanmycin content in the premix and feed.The limit of quantification was 4.8 mg/kg in feed,and the recoveries of hainanmycin in feed and premix were 74.5%~93.8%and 83.2%~101.3%,respectively.It can be used as an effective method for the detection of hainanmycin in feed and premix.

关 键 词：海南霉素 香草醛法 对二甲氨基苯甲醛法 紫外分光光度法 海南霉素钠预混剂 饲料 

分 类 号：O657.32[理学—分析化学]