锦鲤源维氏气单胞菌的鉴定及致病性分析  

Identification and pathogenicity analysis of Aeromonas veronii from Cyprinus carpio var. koi

作　　者：庞月 郭美玲 刘芯睿 薛淑霞 孙金生[1,2] Pang Yue;Guo Meiling;Liu Xinrui;Xue Shuxia;Sun Jinsheng(College of Life Sciences,Tianjin Normal University,Tianjin 300387,China;Tianjin Key Laboratory of Animal and Plant Resistance,Tianjin Normal University,Tianjin 300387,China)

机构地区：[1]天津师范大学生命科学学院,天津300387 [2]天津师范大学天津市动植物抗性重点实验室,天津300387

出　　处：《天津农学院学报》2022年第4期36-42,共7页Journal of Tianjin Agricultural University

基　　金：天津市海水养殖现代农业产业技术体系创新团队(ITTMRS2022006);天津市劳模创新团队(无编号)。

摘　　要：本研究从患病养殖锦鲤体内分离获得一株优势菌株CTJ206。人工感染试验结果表明该菌株为锦鲤致病病原。通过分析菌株CTJ206的生理生化特性及保守基因16SrDNA、gyrB及rpoD的基因序列,鉴定菌株CTJ206为维氏气单胞菌。以维氏气单胞菌人工感染健康锦鲤,结果表明该菌株对锦鲤的半数致死量LD50为1.77×10^(6)CFU/g。分析11种气单胞菌常见毒力基因,结果表明该菌株携带ahyB、ascV、gcaT、act、lip、exu、aerA、fla 8种毒力基因。维氏气单胞菌感染对锦鲤造成肝脏组织细胞坏死及脾脏组织铁血黄素沉积的组织病理变化。药敏试验结果表明,该菌株对阿奇霉素、四环素、多西环素、诺氟沙星、环丙沙星、恩诺沙星等药物高度敏感,对红霉素、万古霉素等已产生耐药性。本研究结果阐释了维氏气单胞菌CTJ206菌株的致病特性,可为锦鲤细菌性疾病的预防和控制提供依据。In this study, a dominant strain CTJ206 was isolated from diseased Cyprinus carpio var. koi. The experimental results of artificial infection showed that the strain CTJ206 was the pathogen of Cyprinus carpio var. koi. By analyzing the physiological and biochemical characteristics of strain CTJ206 and the conserved gene sequences of 16S rDNA, gyrB gene and rpoD gene, we identified the strain CTJ206 as Aeromonas veronii. The healthy Cyprinus carpio var. koi were artificially infected with Aeromonas veronii, and the results showed that the LD50 of the strain to Cyprinus carpio var. koi was 1.77 × 10^(6) CFU/g. We analyzed the common virulence genes of pathogenic bacteria, and the results showed that the strain carried 8 virulence genes of ahyB、ascV、gcaT、act、lip、exu、aer A and fla. Histopathological changes in Cyprinus carpio var. koi caused by Aeromonas veronii infection caused necrosis of liver tissue cells and hemosiderin deposition in spleen tissue. The drug susceptibility test results showed that the strain was highly sensitive to azithromycin, tetracycline, doxycycline, norfloxacin, ciprofloxacin, enrofloxacin and other drugs, and has developed resistance to erythromycin, vancomycin, etc. The results of this study can provide a reference for the prevention and treatment of bacterial diseases of Cyprinus carpio var. koi.

关 键 词：锦鲤 维氏气单胞菌 分离鉴定 药敏试验 毒力基因 

分 类 号：S852.6[农业科学—基础兽医学]