人工感染禽腺病毒血清1型对SPF鸡天然免疫反应的影响  

Effects of artificially infected fowl adenovirus serotype 1 on natural immune response of SPF chickens

作　　者：马得莹[1] 焦亚茹 李雪峰 李慧昕[2] 王芳芳[2] 范佳慧 谢晶晶 MA Deying;JIAO Yaru;LI Xuefeng;LI Huixin;WANG Fangfang;FAN Jiahui;XIE Jingjing(School of Animal Sciences and Technology,Northeast Agricultural University,Harbin 150030,China;National Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China)

机构地区：[1]东北农业大学动物科学技术学院,哈尔滨150030 [2]中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室,哈尔滨150001

出　　处：《东北农业大学学报》2022年第12期35-45,共11页Journal of Northeast Agricultural University

基　　金：黑龙江省自然科学基金项目(TD2021C001);现代农业产业技术体系岗位科学家专项(CARS-40-K18)。

摘　　要：试验旨在探究禽腺病毒血清1型(Fowl adenovirus serotype 1,FAdV-1)感染SPF鸡后产生的天然免疫反应及免疫调控机制。结果表明,感染FAdV-1后,观察组、感染组、对照组SPF鸡均无明显发病和死亡,剖检肌胃和腺胃可见轻微病变。感染初期小肠组织中Toll样受体2(Toll-like receptor 2,TLR2)、5、7、21;禽β-防御素5(Avian β-defensins 5,AvBD5)、8;白细胞介素8(Inter leukin-8,IL-8)、18;MyD88(Myeloid differentiation factor 88,MyD88)、C-Rel及p65基因表达量显著升高(P<0.05)。TLR2、TLR5、AvBD8、IL-8、MyD88、C-Rel和p65在感染后36 h(hour post-infection,hpi)表达趋势一致。感染后期,部分组织中TLR15、AvBD10、AvBD12、IL-8基因表达量显著上调(P<0.05)。试验说明SPF鸡感染FAdV-1初期的先天免疫应答是小肠中通过TLR2、5-MyD88途径产生并调控AvBDs及细胞因子清除病毒。试验为今后可通过外源条件提高禽类天然免疫机能,预防控制FAdV-1及相似病原提供重要理论依据。The purpose of this study was to explore the innate immune response and immune regulation mechanism of SPF chickens infected with Fowl adenovirus serotype 1(FAdV-1). The results showed that there was no obvious morbidity and death in the observation group, infected group and control group of SPF chickens infected with FAdV-1, and slight pathological changes were found in the muscular stomach and glandular stomach. In the early stage of infected, the expressions of Toll-like receptors 2(TLR2), 5, 7, 21, avian β-defensins 5(AvBD5), 8, Inter leukin-8(IL-8), 18, Myeloid differentiation factor 88(My D88), C-Rel and p65 genes in small intestinal tissue were significantly increased. The expression trends of TLR2, TLR5, AvBD8, IL-8, MyD88, C-Rel and p65 were consistent in 36 hpi. In the late stage of infected, the expressions of TLR15, AvBD10, 12 and IL-8 genes were significantly up-regulated in some tissues. The results showed that the innate immune response of SPF chickens infected with FAdV-1 was produced through TLR2-MyD88 and TLR5-MyD88 pathway in the small intestine and regulated AvBDs and cytokines to eliminate the virus. This experiment provided an important theoretical basis for improving the innatel immune function of poultry, and for preventing and controlling FAdV-1 and similar pathogens through exogenous conditions in the future.

关 键 词：禽腺病毒血清1型 SPF鸡 TOLL样受体 禽β防御素 天然免疫反应 

分 类 号：S767.5[农业科学—森林保护学] X172[农业科学—林学]