空心菜WRKY基因家族成员鉴定与表达分析  

Genome-wide Identification and Expression Analysis of WRKY Gene Family in Water Spinach (Ipomoea aquatica)

作　　者：张宇欣 刘征 张唐权 郝园园[1] 成善汉[1] 汪志伟[1] 朱婕[1] 马武强 包文龙 Zhang Yuxin;Liu Zheng;Zhang Tangquan;Hao Yuanyuan;Cheng Shanhan;Wang Zhiwei;Zhu Jie;Ma Wuqiang;Bao Wenlong(Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province,College of Horticulture,Hainan University,Haikou,570228)

机构地区：[1]海南大学园艺学院,海南省热带园艺作物品质调控重点实验室,海口570228

出　　处：《分子植物育种》2023年第1期55-66,共12页Molecular Plant Breeding

基　　金：海南省自然科学基金高层次人才项目(2019RC059);海南省自然科学基金面上项目(320MS008);海南大学高层次人才项目(KYQD(ZR)1935)共同资助

摘　　要：为了更好地了解植物特有的WRKY转录因子在调控空心菜(Ipomoea aquatica)花青素生物合成途径中的作用,利用课题组前期发表的高质量基因组数据,对空心菜WRKY基因家族成员(IaWRKY)进行了全基因组鉴定与表达分析。结果显示,空心菜拥有82个IaWRKY基因。其中,80个IaWRKY基因可以分为3组(Ⅰ组,Ⅱ组和Ⅲ组)。另有2个IaWRKY基因(IaWRKY10和IaWRKY75)没有与拟南芥WRKY基因(AtWRKY75)聚类在一起。此外,Ⅱ组可以进一步分为5个亚组。motif分析显示,同一亚组的IaWRKY蛋白具有相似的motif分布模式。基因结构分析显示,大多数IaWRKY基因拥有3个外显子。染色体定位分析显示,IaⅢWRKY基因不均匀分布在15条染色体和4条Scaffold上。启动子序列分析显示,IaWRKY基因具有多种顺式作用元件。RNA-seq与RT-qPCR结果分析显示,IaWRKY26基因在紫色茎秆空心菜中的转录本丰度是绿色茎秆空心菜中的30倍以上。本研究为探索植物花青素合成机理提供了重要的基因资源。To better understand the role of plant-specific WRKY transcription factors regulating anthocyanin biosynthesis pathway in water spinach(Ipomoea aquatica), we performed genome-wide identification and expression analysis of WRKY gene family in water spinach(IaWRKY) by using the high-quality genomic data previously published by our research group. The results showed that the water spinach possesses 82 IaWRKY genes. Among them,80 IaWRKY genes can be divided into 3 groups(groupⅠ, groupⅡ and groupⅢ). Another two IaWRKY genes(IaWRKY10 and IaWRKY75) were not clustered with Arabidopsis WRKY gene(AtWRKY75). In addition, groupⅡcan be further classified into five subgroups. The analysis of Motif showed that IaWRKY proteins in the same subgroup have similar Motif distribution pattern. The analysis of gene structure showed that the most of IaWRKY genes have 3 exons. The analysis of chromosomal location showed that IaWRKY genes were unevenly distributed on 15 chromosomes and 4 scaffolds. The analysis of promoter sequences showed that IaWRKY genes have multiple cis-acting elements. The analysis of R NA-seq and RT-q PCR showed that the transcript abundances of IaWRKY26 gene in purple-stemmed water spinach was 30-fold higher than that in green-stemmed water spinach.This study provides important gene resources for exploring the mechanism of anthocyanin synthesis in plants.

关 键 词：空心菜 WRKY转录因子 生物信息学 表达分析 花青素生物合成 

分 类 号：S636.9[农业科学—蔬菜学]