苍艾挥发油对左心室重构大鼠的改善作用研究  

Ameliorative Effects of Cang-ai Volatile Oil on Left Ventricular Remodeling in Rats

作　　者：李亥辰 黎梅 张明星[1] 黄睿珏 温小娟 陈柏君 郜发宝[3] 邬颖华 LI Hai-chen;LI Mei;ZHANG Ming-xing;HUANG Rui-jue;WEN Xiao-juan;CHEN Bo-jun;GAO Fa-bao;WU Ying-hua(Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China;Department of Otorhinolaryngology,Rongchang Hospital of Traditional Chinese Medicine,Chongqing 402460,China;Department of Radiology,West China Hospital,Sichuan University,Chengdu 610041,China;College of Basic Medicine,Yunnan University of Chinese Medicine,Kunming 650500,China)

机构地区：[1]成都中医药大学附属医院,成都610075 [2]重庆市荣昌区中医院五官科,重庆402460 [3]四川大学华西医院放射科,成都610041 [4]云南中医药大学基础医学院,昆明650500

出　　处：《四川大学学报（医学版）》2023年第1期128-135,共8页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金项目(No.81930046)资助。

摘　　要：目的采用7T心脏磁共振组织追踪成像(CMR-TT)评估苍艾挥发油(CAVO)对异丙肾上腺素(ISO)所诱导的左心室重构(LVR)模型大鼠的改善作用,并初步探讨其对LVR内皮功能障碍的影响。方法将35只SD雄性大鼠随机分成两组,即正常对照组(8只)和实验组(27只),通过ISO 10 mg·kg-1·d-1连续10 d皮下多点注射建立LVR大鼠模型。造模结束后将实验组存活大鼠(24只)再次随机分为空白实验组、苍艾挥发油组、麝香保心丸组(每组8只),各组连续给予相应药物或等量生理盐水灌胃28 d,分别在造模结束后和灌胃结束后行7T CMR电影序列扫描,并应用后处理软件CVI42进行图像分析,对比各组大鼠用药前后左心室结构和功能参数的变化。磁共振扫描结束后处死大鼠,摘取心脏行病理学检查,ELISA检测血清生化指标。结果苍艾挥发油可明显提高LVR大鼠的左心室射血分数和整体心肌应变参数,降低左心室容积、质量和血清中内皮功能指标水平,同时病理染色显示心肌细胞肥大、坏死以及间质纤维化也得到明显改善。结论CAVO通过对心肌血管内皮功能的调控,可明显改善LVR大鼠心功能,延缓心室重构进程,对大鼠心脏结构和功能具有一定的保护作用。Objective To evaluate with 7T cardiac magnetic resonance tissue tracking imaging(CMR-TT)the ameliorative effect of Cang-ai volatile oil(CAVO)on left ventricular remodeling(LVR)in rats induced by isoproterenol(ISO),and to make preliminary investigation into CAVO’s effects on endothelial dysfunction in LVR.Methods A total of 35 healthy male Sprague-Dawley(SD)rats were randomly assigned to two groups,the experimental group(n=27)and the normal control group(n=8).The rat model of LVR was established by subcutaneous injection of ISO solution at 10mg·kg-1·d-1at multiple sites for 10 consecutive days.After modeling was completed,the surviving rats(n=24)in the experimental group were then randomly assigned to the blank experimental group,CAVO group,and Shexiang Baoxin pill(SXBXP)group(n=8 in each group).Rats in each group were given via gavage the corresponding intervention medicine or an equivalent amount of normal saline solution for 28 consecutive days.At the end of modeling and intragastric intervention,7T CMR cine sequence scanning was conducted to collect data.Then,post-processing software CVI42 was used to analyze the images and to compare and contrast the changes in the parameters of left ventricular cardiac function and myocardial strain in each group before and after the administration of the medication.The rats were sacrificed after MRI scanning,and their hearts were harvested for pathological examination.The levels of serum biochemical indicators were measured by enzyme-linked immunosorbent assay(ELISA).Results CAVO significantly increased LV ejection fraction and overall myocardial strain parameters in LVR rats,while it decreased LV volume,mass,and serum levels of endothelial function indicators in LVR rats.In addition,pathological staining showed marked improvements in the hypertrophy,necrosis and interstitial fibrosis of cardiomyocytes.Conclusion Through the regulation of myocardial vascular endothelial function,CAVO can significantly improve cardiac functions in LVR rats,delay the process of ventricula

关 键 词：苍艾挥发油 7T心脏磁共振 组织追踪成像 左心室重构 

分 类 号：R285.5[医药卫生—中药学]