山羊角抗惊厥活性评价及其对小鼠脑内神经递质的影响  

Anti-convulsant Activity of Goat Horn and Its Effect on Neurotransmitters in Mouse Brain

作　　者：朱昭颖 武文星 赵晶晶[1] 刘睿[1] 郭盛[1] 段金廒[1] ZHU Zhaoying;WU Wenxing;ZHAO Jingjing;LIU Rui;GUO Sheng;DUAN Jin'ao(National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,and National Administration of Traditional Chinese Medicine Key Laboratory for Chinese Medicine Resources Recycling Utilization,Nanjing University of Chinese Medicine,Nanjing 210023,China)

机构地区：[1]南京中医药大学中药资源产业化与方剂创新药物国家地方联合工程研究中心,江苏省中药资源产业化过程协同创新中心,国家中医药管理局中药资源循环利用研究重点实验室,南京210023

出　　处：《中国实验方剂学杂志》2023年第3期133-142,共10页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81873189,81973450);江苏省高校自然科学研究重大项目(18KJA360006)。

摘　　要：目的:考察山羊角抗惊厥活性及其对模型小鼠脑内主要神经递质含量变化的影响。方法:选择小鼠120只随机分为6组,分别为空白组、模型组、艾司唑仑组(1 mg·kg^(-1))、羚羊角组(0.2 g·kg^(-1))、山羊角低剂量组(1.25 g·kg^(-1))、山羊角高剂量组(2.5 g·kg^(-1))。灌胃给药1次,灌胃体积均为10 mL·kg^(-1),空白组及模型组给予同体积0.2%羧甲基纤维素钠(CMC-Na)。小鼠给药后2 h给予阈值电刺激1次,以后肢出现强直性惊厥为观察指标,计算强直性惊厥恢复期、惊厥发生率及死亡率;采用超高效液相色谱-串联质谱法(UPLC-MS/MS)分析小鼠脑组织主要神经递质含量变化。结果:灌胃给予小鼠山羊角粉2 h后可以缩短惊厥恢复期,与模型组比较可以显著降低惊厥发生率和死亡率(P<0.05),抗惊厥效果由强到弱依次为山羊角高剂量组>羚羊角组>山羊角低剂量组;UPLC-MS/MS分析显示,与模型组比较,山羊角粉低剂量组可明显降低脑组织中谷氨酸含量(P<0.05),山羊角粉高、低剂量组可明显降低脑组织中天冬氨酸含量(P<0.05);山羊角低剂量组和羚羊角粉组明显提高甘氨酸含量(P<0.05);山羊角粉高、低剂量组和羚羊角粉可明显提高γ-氨基丁酸、5-羟色胺含量(P<0.05)。。结论:研究结果表明,山羊角对电惊厥小鼠模型具有抗惊厥作用,其作用机制可能为通过调节脑组织神经递质的含量变化改善其惊厥的发生发展。Objective:To investigate the anti-convulsant activity of goat horn and its effect on the main neurotransmitters in the brain of model mice. Method:A total of 120 mice were randomly divided into a blank group,a model group,a positive drug group(estazolam, 1 mg·kg^(-1)),a Saigae Tataricae Cornu(STC,0.2 g·kg^(-1))group,and low-(1.25 g·kg^(-1))and high-dose(2.5 g·kg^(-1))goat horn groups. Drugs were administered by gavage once at 10 mL·kg^(-1). Those in the blank group and the model group were given the same volume of 0.2% sodium carboxymethyl cellulose(CMC-Na). The mice underwent threshold electrical stimulation once 2 h after administration. Ankylosing convulsion in the hind limbs of mice was used as the index to calculate the recovery period, convulsion rate, and mortality of ankylosing convulsion. Ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was established to analyze the changes in the content of the main neurotransmitters in the brain tissues of mice. Result: Two hours after intragastric administration of goat horn powder to mice,the recovery period of convulsion could be shortened and the convulsion rate and mortality were reduced(P<0.05). In terms of anti-convulsant effect,the groups were ranked as follows:high-dose goat horn group > STC group > low-dose goat horn group. UPLC-MS/MS analysis showed that compared with the results in the model group,goat horn powder and STC decreased the content of glutamic acid and aspartic acid(P<0.05) and increased the content of glycine, γ-aminobutyric acid, and 5-hydroxytryptamine(P<0.05). Conclusion: The results showed that goat horn had a significant anticonvulsant effect on the electroshock-induced convulsion mouse model,and the underlying mechanism was presumably attributed to the improvement of the development of convulsion by regulating the changes in neurotransmitter content in brain tissues.

关 键 词：山羊角 电惊厥模型 神经递质 超高效液相色谱-串联质谱法 

分 类 号：R285[医药卫生—中药学] R289[医药卫生—中医学] R22R2-031R33