果糖饮水对小鼠代谢和学习记忆影响的性别差异研究  

Gender differences of effects of fructose drinking water on metabolism,learning and memory in mice

作　　者：梁宁静 莫娇 唐婷婷 杜秉欣 丁欢 王新航 陆彩玲 唐深 李习艺 Liang Ningjing;Mo Jiao;Tang Tingting;Du Bingxin;Ding Huan;Wang Xinhang;Lu Cailing;Tang Shen;Li Xiyi(Department of Nutrition and Food Hygiene,School of Public Health,Guangxi Medical University,Nanning 530021,China;Department of Immunology,School of Basic Medicine,Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学公共卫生学院营养与食品卫生学系,南宁530021 [2]广西医科大学基础医学院免疫学教研室,南宁530021

出　　处：《广西医科大学学报》2022年第12期1937-1944,共8页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.82160612)。

摘　　要：目的:探讨果糖饮水对小鼠代谢和学习记忆影响的性别差异及产生差异的可能机制。方法:选取6~8周龄C57BL/6N雌、雄小鼠适应性饲养1周,按性别随机分为4组,每组6只,分别为雄性对照组、雄性果糖组、雌性对照组和雌性果糖组,其中对照组小鼠给予标准饲料加普通饮用水,果糖组给予标准饲料加30%果糖饮用水,每周记录小鼠体重。持续喂养11周后用Morris水迷宫实验测试小鼠空间记忆能力。喂养12周处死小鼠,取动物血清、肝脏和内脏脂肪;检测血清天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平;肝组织行苏木精—伊红(HE)染色、油红O染色,观察肝脏组织病理学变化;RT-qPCR检测肝脏ChREBP、SREBP-1c、ACC1、CPT-1、FGF21基因表达。结果:喂养12周后,雌性果糖组体重、肝重及内脏脂肪重量显著高于雌性对照组(P<0.05),而雄鼠体重、肝重及内脏脂肪重量不受果糖影响;与对照组比较,雌、雄果糖组空腹血糖均升高(P<0.05),雌性果糖组较雄性果糖组增加更为显著;与相应对照组相比,雌、雄果糖组血清AST、ALT水平未发生改变(P>0.05),雌性果糖组血清TC、HDL-C、LDL-C水平显著升高(P<0.05),雄性果糖组血清TG、LDL-C水平显著升高(P<0.01);肝组织病理结果显示,与相应对照组相比,雌性果糖组小鼠的肝脏出现了明显的脂质沉积、炎症细胞浸润等改变;水迷宫结果显示,与相应对照组相比,雌性果糖组在第4天和第5天的逃避潜伏期显著延长(P<0.05),第6天穿越平台次数及在目标象限停留时间显著减少(P<0.05);雄性果糖组未发生显著变化(P>0.05);与相应对照组相比,小鼠的平均泳速没有显著变化(P>0.05);脂肪生成基因ChREBP、SREBP-1c、ACC1表达在雌、雄小鼠中没有变化(P>0.05);脂肪酸氧化关键基因CPT-1仅在雌鼠中表达降低(P<0.01)Objective:To explore the gender differences and possible mechanisms of the effects of fructose drinking water on metabolism,learning and memory in mice.Methods:C57BL/6N male and female mice aged 6 to 8 weeks were bred for 1 week and were randomly divided respectively into 4 groups according to gender with 6 animals in each group.male control group,male fructose group,female control group and female fructose group.The mice in the control group were given standard diet plus ordinary drinking water,and the mice in the fructose group were given standard diet plus 30%fructose drinking water,with body weight recorded weekly.Morris water maze test was used to test the spatial memory ability of mice after continuous feeding for 11 weeks.The mice were sacrificed after 12 weeks of feeding,and the serum,liver and visceral fat of the animals were collected.The levels of serum aspartate aminotransferase(AST),alanine aminotransferase(ALT),total cholesterol(TC),triglyceride(TG),high density lipoprotein cholesterol(HDL-C)and low density lipoprotein cholesterol(LDL-C)were detected.Liver tissues were stained with HE and oil red O to observe histopatho-logical changes of liver.The expressions of ChREBP,SREBP-1c,ACC1,CPT-1 and FGF21 in liver were detected by real-time fluorescence quantitative polymerase chain reaction(RT-qPCR).Results:After 12 weeks of feeding,the body weight,liver weight and visceral fat weight of female fructose group were significantly higher than those of female control group(P<0.05),while the body weight,liver weight and visceral fat weight of male rats were not affected by fructose.Compared with the control group,the fasting blood glucose in both male and female fructose groups increased(P<0.05),and the female fructose group increased more significantly than the male fructose group.Compared with their control groups,serum AST and ALT were not changed in male and female fructose groups(P>0.05);the serum levels of TC,HDL-C and LDL-C in female fructose group significantly increased(P<0.05),and the serum levels o

关 键 词：果糖饮水 性别差异 糖脂代谢 学习记忆 

分 类 号：R985.2[医药卫生—药品]