基于生物信息学分析筛选银屑病的关键基因  

Screening of Key Genes in Psoriasis Based on Bioinformatics Analysis

作　　者：任静静[1] 石瑛[2] 智艳芳[1] 杨博[1] 李肖甫[1] REN Jing-jing;SHI Ying;ZHI Yan-fang;YANG Bo;LI Xiao-fu(Department of Transfusion,the Third Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,Henan,China;Department of Clinical Laboratory,the Third Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,Henan,China)

机构地区：[1]郑州大学第三附属医院输血科,河南郑州450052 [2]郑州大学第三附属医院检验科,河南郑州450052

出　　处：《医学信息》2022年第24期1-6,共6页Journal of Medical Information

基　　金：河南省高等学校重点科研计划项目(编号:19A320055)。

摘　　要：目的利用生物信息学方法筛选银屑病的关健基因及所在信号通路,探讨其发病机制。方法从开源性基因表达综合数据库(NCBI-GEO)中获取3个银屑病皮损和非皮损活检样本的相关数据集。利用R语言GEO Query和Limma包筛选3个数据集中差异基因(DEGs),通过R语言clusterProfiler包对DEGs进行生物学过程、信号通路分析,最后通过Cytoscape3.6.0软件做出蛋白-蛋白互作网络分析并筛选关键基因。结果3个数据集取交集共获得共计297个共同差异基因,其中184个上调DEGs和113个下调DEGs;筛选出的10个关键基因,均为上调表达基因;KEGG信号通路主要富集于IL-17信号通路、病毒蛋白与细胞因子/受体互作通路以及趋化因子信号通路;GOBP主要富集在趋化因子的生物学过程中。结论趋化因子及IL-17信号通路可能在银屑病的发生发展中起着重要作用,为其分子机制、诊断和生物学治疗提供指导。Objective To screen the key genes and signaling pathways of psoriasis by bioinformatics methods,and to explore its pathogenesis.Methods The relevant data sets of 3 psoriatic lesion and non-lesion biopsy samples were obtained from the open source gene expression comprehensive database(NCBI-GEO).The R language GEO Query and Limma package were used to screen differentially expressed genes(DEGs)in three datasets.The R language clusterProfiler package was used to analyze the biological processes and signaling pathways of DEGs.Finally,the protein-protein interaction network was analyzed by Cytoscape 3.6.0 software and the key genes were screened.Results A total of 297 common differential genes were obtained from the intersection of the three datasets,including 184 up-regulated DEGs and 113 down-regulated DEGs;the10 key genes screened were all up-regulated genes.The KEGG signaling pathway was mainly enriched in IL-17 signaling pathway,virus protein and cytokine/receptor interaction pathway and chemokine signaling pathway.GOBP was mainly enriched in the biological processes of chemokines.Conclusion Chemokines and IL-17 signaling pathway may play an important role in the occurrence and development of psoriasis,and provide guidance for its molecular mechanism,diagnosis and biological treatment.

关 键 词：银屑病 生物信息学 IL-17信号通路 趋化因子 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]