慢性应激联合孤养对自发性高血压大鼠行为学、血清中5-HT与海马区神经元的影响  

Effects of Chronic Stress Combined with Solitary Rearing on Behavior,Serum 5-HT and Hippocampal Neurons in Spontaneously Hypertensive Rats

作　　者：陈蕾 刘叶倩 赵红霞 李弘 吴碧茹 曾勇[1] 马丹凤[4] 陈春茗 刘林[1] 任卫琼[1] 易健[1] CHEN Lei;LIU Yeqian;ZHAO Hongxia;LI Hong;WU Biru;ZENG Yong;MA Danfeng;CHEN Chunming;LIU Lin;REN Weiqiong;YI Jian(the First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China;Anhui No.2 Provincial People’s Hospital,Hefei 230041,China;Technology Innovation Center,Hunan Academy of Chinese Medicine,Changsha 410208,China;Hunan Children’s Hospital,Changsha 410007,China)

机构地区：[1]湖南中医药大学第一附属医院,长沙410007 [2]安徽省第二人民医院,合肥230041 [3]湖南中医药大学科技创新中心,长沙410208 [4]湖南省儿童医院,长沙410007

出　　处：《实验动物科学》2022年第5期1-6,共6页Laboratory Animal Science

基　　金：湖南省自然科学基金项目(2021JJ80065);中药粉体与创新药物省部共建国家重点实验室培育基地开放基金(21PTKF1010);湖南省卫生计生委项目(20200312);湖南省教育厅项目(19C1415);湖南省教育厅项目(20B448);湖南省中管局项目(2021072);国家自然科学基金项目(82104842);湖南省卫生健康委项目(202103011378)。

摘　　要：目的 观察慢性不可预知温和应激(CUMS)联合孤养对自发性高血压大鼠(SHR)行为学、血清中5-HT与海马区神经元的影响。方法 SHR大鼠20只,随机均分为高血压对照组和模型组,另设同数Sprague-Dawley(SD)大鼠为空白组。模型组以CUMS联合孤养的方法干预5周,其余组正常饲养。分别在造模14 d及造模35 d后测量各组大鼠尾动脉血压,进行旷场实验,糖水消耗实验;造模结束后进行强迫游泳实验,ELISA法检测血清中5-HT含量,HE和尼氏染色观察海马神经元的活性、排列情况以及神经元尼氏小体的数量。结果 与空白组比较,高血压对照组大鼠收缩压明显升高(P<0.01),体质量增长率明显增大(P<0.01),水平活动与直立次数、糖水消耗率及不动时间、血清中5-HT含量无统计学差异(P>0.05),海马神经元细胞形态圆整,排列整齐,尼氏小体多且密集;模型组大鼠收缩压明显升高(P<0.01),体质量增长率无统计学差异(P>0.05),站立与水平活动次数、糖水消耗率明显降低(P<0.05,P<0.01),不动时间明显增加(P<0.01),血清中5-HT含量明显降低(P<0.01),海马神经元排列疏松,尼氏体凋亡。与高血压对照组比较,模型组大鼠收缩压无明显升高(P>0.05),体质量增长率、站立与水平活动次数、糖水消耗率明显降低(P<0.01),不动时间明显增加(P<0.01),血清中5-HT含量明显降低(P<0.01),海马神经元排列疏松,尼氏小体凋亡。结论 采用慢性不可预知温和应激(CUMS)联合孤养诱导SHR大鼠行为学、血清中5-HT与海马区神经元呈现抑郁样改变。Objective To observe the effects of chronic unpredictable mild stress(CUMS)combined with solitary rearing on the behavior, serum 5-HT and neurons in the hippocampus of spontaneously hypertensive rats(SHR).Method Twenty SHR rats were randomly divided into SHR group and model group, and the same number of Sprague-Dawley(SD) rats were set as the control group.Predictive stress(CUMS) combined with solitary rearing was used to intervene for 5 weeks, and the rest of the groups were fed normally.The caudal arterial blood pressure of rats in each group was measured on the 14 th day and at the end of modeling, respectively. Open-field experiment and sugar water consumption experiment were performed. Forced swimming experiment was conducted after modeling, 5-HT content in serum was detected by ELISA, and activity and arrangement of hippocampal neurons and the number of neurons’ Nissl bodies were observed by HE staining and Nissl staining.Result Compared with the control group, the blood pressure of SHR group increased significantly(P<0.01), body weight growth rate increased obviously(P<0.01), the level of activity and the number of upright, sugar water consumption rate and forced swimming fixed time, 5-HT content there was no significant difference(P>0.05), roundness, hippocampal neurons form order, nissl bodies and more dense;Systolic blood pressure of model group was significantly increased(P<0.01), body weight growth rate was not significantly different(P>0.05), horizontal activity and upright times, sugar water consumption rate were significantly decreased(P<0.05, P<0.01), forced swimming immobile time was significantly increased(P<0.01), 5-HT content was significantly decreased(P<0.01).The arrangement of hippocampal neurons was loose and the nite body was apoptotic.Compared with SHR group, systolic blood pressure in model group was not significantly increased(P>0.05), body weight growth rate, horizontal activity and upright times, sugar water consumption rate were significantly decreased(P<0.01), the immobile time o

关 键 词：高血压并发抑郁 慢性不可预知温和应激 孤养 自发性高血压大鼠 模型 

分 类 号：Q95-3[生物学—动物学]