转基因抗虫耐除草剂玉米瑞丰125 Cry1Ab/Cry2Aj杀虫蛋白的时空表达分析  

Spatio-temporal Expression of Cry1Ab/Cry2Aj Insecticidal Protein in Genetically Modified Maize Ruifeng 125 with Stacked Insect and Herbicide Resistance Traits

作　　者：李东阳 肖冰 王晨尧 杨现明[2] 梁晋刚 吴孔明[2] LI Dong-yang;XIAO Bing;WANG Chen-yao;YANG Xian-ming;LIANG Jin-gang;WU Kong-ming(Development Center of Science and Technology,Ministry of Agriculture and Rural Affairs,Beijing 100176;State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193;State Key Laboratory of Cotton Biology,Institute of Cotton Research,Chinese Academy of Agricultural Sciences,Anyang 455000;Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081)

机构地区：[1]农业农村部科技发展中心,北京100176 [2]中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京100193 [3]中国农业科学院棉花研究所棉花生物学国家重点实验室,安阳455000 [4]中国农业科学院农业资源与农业区划研究所,北京100081

出　　处：《生物技术通报》2023年第1期31-39,共9页Biotechnology Bulletin

基　　金：转基因生物新品种培育重大专项(2019ZX08012-004);农业科研杰出人才培养计划(2021年)。

摘　　要：玉米是我国最重要的粮食作物之一,虫害会造成严重的品质和产量损失,转Bacillus thuringiensis(Bt)基因抗虫玉米为玉米害虫的防治提供了新途径。转Bt基因抗虫玉米的抗虫性与外源杀虫蛋白表达量具有密切的关系,明确Bt杀虫蛋白在不同生育期和不同器官中的表达量,对害虫的综合防治和农业转基因生物安全管理具有重要意义。连续两年采用酶联免疫吸附法(ELISA)测定了9个地点种植的瑞丰125不同器官中Cry1Ab/Cry2Aj杀虫蛋白表达量,对比分析了玉米拔节期(V6-V8)叶片、抽雄期(VT)雄穗、吐丝期(R1)叶片和花丝、成熟期(R4)叶片和籽粒的Bt杀虫蛋白含量,结果表明Bt杀虫蛋白表达量在不同玉米器官中差异较大。2年9个地点的数据整体上呈现出叶片中Cry1Ab/Cry2Aj杀虫蛋白表达量较高,而籽粒中Cry1Ab/Cry2Aj杀虫蛋白表达量较低的规律。9个地点种植的瑞丰125的Cry1Ab/Cry2Aj杀虫蛋白表达量有所差异,但差异整体较小。Maize is one of the most important food crops in China,but pests reduce the quality and yield of maize production.Genetically modified(GM)insect resistant maize expressing Bacillus thuringiensis(Bt)toxins are new tools for integrated pest management.The insecticidal effect of Bt-transgenic maize is closely related to the expression of exogenous insecticidal protein.Evaluating the expression of Bt insecticidal protein in different growth stages and tissues is of significance to the integrated pest management and biosafety management of agricultural GM organisms.In this paper,the expression of Cry1Ab/Cry2Aj insecticidal protein in different tissues of Ruifeng 125 planted in 9 places in two years was determined by ELISA.The Bt insecticidal protein expressions in the leaves at jointing stage(V6-V8),tassels at tasseling stage(VT),leaves and filaments at silking stage(R1),leaves and grains at maturity stage(R4)were comparatively analyzed.It was found that the expressions of Bt insecticidal protein in different tissues of maize varied largely.The results showed that the expression of Cry1Ab/Cry2Aj insecticidal protein in the leaves was higher than that in the kernels.The expressions of Cry1Ab/Cry2Aj insecticidal protein of Ruifeng 125 from 9 different places were different,but the overall difference was slight.

关 键 词：转基因抗虫玉米 瑞丰125 Cry1Ab/Cry2Aj杀虫蛋白 时空表达 

分 类 号：S513[农业科学—作物学]