转基因玉米HGK60在不同遗传背景下抗虫性鉴定及农艺性状分析  

Insect Resistance Identification and Agronomy Traits Analysis of Transgenic Maize HGK60 with Different Genetic Backgrounds

作　　者：李鹏程 张明俊 王银晓 李香银 李圣彦[1] 郎志宏[1] LI Peng-cheng;ZHANG Ming-jun;WANG Yin-xiao;LI Xiang-yin;LI Sheng-yan;LANG Zhi-hong(Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081)

机构地区：[1]中国农业科学院生物技术研究所,北京100081

出　　处：《生物技术通报》2023年第1期40-47,共8页Biotechnology Bulletin

基　　金：国家转基因生物新品种培育重大专项(2016ZX08003-001);中国农业科学院科技创新工程(CAAS-ZDRW202004)。

摘　　要：为研究转基因玉米HGK60在不同遗传背景下遗传稳定性和抗虫效果,利用转Bt cry1Ah基因的转基因玉米HGK60为供体,通过回交转育的方式将cry1Ah基因分别导入玉米自交系郑58、昌7-2、lx05-4、lx03-2,获得转基因玉米自交系HGK60-郑58、HGK60-昌7-2、HGK60-lx03-2、HGK60-lx05-4,并杂交获得HGK60-郑单958(HGK60-郑58×HGK60-昌7-2)和HGK60-鲁单9066(HGK60-lx05-4×HGK60-lx03-2),转化体特异性PCR证明cry1Ah基因已转入不同遗传背景玉米中,ELISA检测不同遗传背景转基因玉米叶片中Cry1Ah蛋白表达情况,结果表明在不同遗传背景玉米自交系和杂交种中Cry1Ah蛋白表达没有显著差异;田间人工接虫和室内玉米螟抗虫性鉴定结果表明,不同遗传背景的转基因玉米高抗玉米螟,室内接虫后4 d幼虫死亡率达到100%;对不同遗传背景转基因玉米HGK60进行农艺性状分析,结果显示与受体对照玉米相比,两者之间农艺性状没有显著差异,转基因玉米HGK60可用于抗虫玉米品种的选育。In order to study the genetic stability and insect resistance of transgenic maize HGK60 under different genetic backgrounds,HGK60 transgenic maize harboring Bt cry1Ah gene was used as donor,and the cry1Ah gene was introduced into inbred line Zheng 58,Chang 7-2,lx05-4 and lx03-2 by successive backcrossing respectively,and transgenic maize inbred line HGK60-Zheng 58,HGK60-Chang 7-2,HGK60-lx03-2,and HGK60-lx05-4 were acquired.The hybrid line HGK60-zhengdan 958(HGK60-Zheng 58 with HGK60-Chang 7-2)and HGK60-Ludan 9066(HGK60-lx03-2 with HGK60-lx05-4)were obtained by crossing.The foreign cry1Ah gene was confirmed to be transferred into the maize with different genetic background by event-special PCR detection.The expressions of Cry1Ah proteins in the transgenic maize leaves of the inbred and hybrid lines under different genetic backgrounds were insignificantly different by ELISA detection.Bioassay in field and in laboratory showed that the transgenic maize with different genetic backgrounds had same high resistance to Asia corn borer,and the mortality rate reached 100%at 4 d after indoor inoculation.The agronomic traits of the transgenic maize HGK60 with different genetic backgrounds demonstrated that there was no significant difference in agronomic traits between the transgenic maize and the non-transgenic control maize,and transgenic maize HGK60 may be used for the breeding of insect-resistant maize varieties.

关 键 词：转基因玉米 cry1Ah基因 不同遗传背景 抗虫性 农艺性状 

分 类 号：S513[农业科学—作物学]