基于转录组测序的沟叶结缕草花序发育相关基因筛选及分析  

Screening and Analysis of Genes Related to Inflorescence Development of Zoysia matrella Based on RNA-seq

作　　者：蔡宇 周认 柴明良[1] Cai Yu;Zhou Ren;Chai Mingiang(College of Agriculture and Biotechnology,ZhejiangUniversity,Hangzhou,310058)

机构地区：[1]浙江大学农业与生物技术学院,杭州310058

出　　处：《分子植物育种》2022年第24期8005-8016,共12页Molecular Plant Breeding

基　　金：国家自然科学基金项目(30771522;31672195)资助。

摘　　要：沟叶结缕草是一种优良的暖季型草坪草,生态适应性强,应用广泛。但其花果期明显的花序影响草坪外观质量,也影响其营养生长,频繁修剪增加成本且对环境不友好。本研究通过Illumina高通量测序平台对沟叶结缕草多穗突变体进行转录组测序,以野生型植株为对照,结果共得到42.71 Gb Clean Data,各样品Q30均不小于95.15%,共发掘2 647个新基因,其中有979个新基因在不同数据库中得到功能注释。共鉴定出4 790个DEGs(差异表达基因),其中1 736个基因上调,3 054个基因下调,通过GO数据库进行功能注释,共有2 840个基因被注释到51个GO项中,其中主要富集于代谢过程、细胞过程、单有机体过程、膜、细胞组分、细胞、结合、催化活性、转运体活性等功能。KEGG分析显示较多基因富集在植物-病原互作通路、碳代谢、植物激素通路、固碳型蔗糖代谢、光感应生物体碳固定、生物合成氨基酸、剪接体、内质网蛋白质加工通路中,富集最为显著的通路是卟啉与叶绿素代谢、碳代谢、光合作用-天线蛋白和光合生物中的碳固定,表明沟叶结缕草的花序发育过程受多个代谢通路共同调控。通过WGCNA分析推测EHD1基因和MADS14基因是沟叶结缕草花序发育的相关基因,为后续通过基因工程手段减少花序产生、提高成坪质量提供可依据的基础数据。Zoysia matrella, an excellent warm-season turfgrass, has strong ecological adaptability and is widely used. However, the conspicuous inflorescences affected the appearance of the lawn and vegetative growth of the turfgrass in the flowering and heading period. Frequent pruning increases costs and is environmentally unfriendly.In this study, the transcriptome sequencing of the multi-spike mutants of Zoysia matrella was carried out by Illumina high-throughput sequencing platform, and the wild type plants were used as the control. A total of 42.71 Gb Clean Data were obtained, and the percentage of Q30 bases in each sample was not less than 95.15%. A total of 2 647 new genes were discovered. Among them, 979 new genes were functionally annotated in different databases. A total of 4 790 DEGs(differentially expressed genes) were identified, among which 1 736 genes were up-regulated and 3 054genes were down-regulated. Functional annotation was performed through GO database, and a total of 2 840 genes were annotated into 51 GO items. It is mainly enriched in metabolic process, cellular process, single-organism process, membrane, cell part, cell, binding, catalytic activity, transporter activity and other functions. KEGG analysis showed that more genes were enriched in plant-pathogen interaction, carbon metabolism plant hormone signal transduction, MAPK signaling pathway-plant, starch and sucrose metabolism, biosynthesis of amino acids, spliceosome, and protein processing in endoplasmic reticulum. The most significantly enriched pathways were porphyrin and chlorophyll metabolism, carbon metabolism, photosynthesis-antenna proteins and carbon fixation in photosynthetic organisms, indicating that the regulation of inflorescence development in Zoysia matrella is the result of multiple metabolic pathways. Through WGCNA analysis, EHD1 gene and MADS14 gene were inferred to be related genes for inflorescence development of Zoysia matrella, which could provide basic data for reducing inflorescence production and improving turf quali

关 键 词：沟叶结缕草 草坪草抽穗 转录组 花序发育 诱变育种 

分 类 号：S688.4[农业科学—观赏园艺]