三个棉种中LACS家族基因鉴定及其对盐胁迫的响应  

Genome-wide Identification of LACS Family Members in Three Cotton Species and Their Responses to Salt Stress

作　　者：盖文瑜 孙华 张海君 宋宪亮[2] 盖树鹏[1] 袁延超 Gai Wenyu;Sun Hua;Zhang Haijun;Song Xianliang;Gai Shupeng;Yuan Yanchao(College of Life Science,Qingdao Agricultural University,Qingdao,266109;Agronomy College,Shandong Agricultural University,Taian,271018)

机构地区：[1]青岛农业大学生命科学学院,青岛266109 [2]山东农业大学农学院,泰安271018

出　　处：《分子植物育种》2022年第24期8017-8028,共12页Molecular Plant Breeding

基　　金：山东省自然科学基金项目(ZR2020QC160);国家大学生创新创业训练计划项目(S202010435003);山东省农业良种工程项目(2020LZGC002);山东省现代农业产业技术研究体系项目(SDAIT-03-03/05);青岛农业大学高层次人才科研基金项目(663/1120077)共同资助。

摘　　要：长链脂酰辅酶A合酶(LACS)是脂酰辅酶A合酶中的一个亚组,能将长链或超长链脂肪酸转化为相应的硫酯,参与多种脂质相关过程。植物中LACSs参与角质层合成,影响着植物对各种环境胁迫的耐受性,包括干旱、盐胁迫、缺氧反应以及生物胁迫等。棉花是中国天然纤维、植物油和植物蛋白的重要来源之一。本研究利用生物信息学方法在亚洲棉、雷蒙德棉和陆地棉中分别鉴定到11个、11个和22个LACSs基因,并将其分为7个亚组,也对其理化性质、基因结构、保守结构域及保守基序等进行了分析。共线性分析表明,四倍体陆地棉的GhLACSs基因家族是由二倍体种的LACSs整合形成的。顺式作用元件分析发现大部分GhLACSs启动子区域具有响应激素和胁迫的顺式作用元件;转录表达谱及RT-qPCR结果显示,GH_A01G1967、GH_A02G1129、GH_D03G0938和GH_D06G0458的表达受到盐胁迫的诱导,推测这四个基因在陆地棉盐胁迫响应过程中发挥重要作用。这些结果为后续进一步研究GhLACSs的耐盐功能以及培育耐盐新品种提供了重要的依据。Long-chain acyl-CoA synthase(LACS) is a subgroup of acyl-CoA synthase, which can convert long-chain or very-long-chain fatty acids into corresponding thioesters, participating in several lipid-related processes. In plants, LACSs are involved in cuticle synthesis, which affects their tolerance to various stresses, including drought,salt stress, hypoxia and biotic stress. Cotton is one of the important sources of natural fiber, vegetable oil and plant protein in China. In this study, 11, 11 and 22 LACS genes were identified in G. arboretum, G. raimondii and G.hirsutum by bioinformatics method, and they were divided into seven subgroups. Their physicochemical properties,gene structure, conserved domains and conserved motifs were also analyzed. The collinearity analysis showed that the GhLACS gene family of tetraploid upland cotton was formed by LACSs integration of diploid species. The cis-elements analysis showed that most GhLACS genes had cis-elements that responded to different phytohormones and stresses. Transcription expression profiles and RT-qPCR results showed that GH_A01G1967, GH_A02G1129,GH_D03G0938 and GH_D06G0458 could respond to salt stress, which suggested that these four genes played an important role in the process of salt tolerance in upland cotton. These results provide an important basis for further research on the salt-tolerant function of GhLACSs and cultivation of new salt-tolerant varieties.

关 键 词：陆地棉 LACS基因家族 生物信息学 盐胁迫 

分 类 号：S562[农业科学—作物学]