微生境下小叶章基因组基于毛细管电泳的MSAP分析  

Analysis of Methylation-sensitive Amplified Polymorphism in Deyeuxia angustifolia Based on Capillary Electrophoresis

作　　者：张玉[1] 冷海楠[1] 王继丰[1] 徐明怡[1] 倪红伟 杨轶华[1] Zhang Yu;Leng Hainan;Wang Jifeng;Xu Mingyi;Ni Hongwei;Yang Yihua(Institute of Natural Resource and Ecology,Heilongjiang Academy of Sciences,Harbin,150040;Heilongjiang Academy of Forestry,Harbin,150081)

机构地区：[1]黑龙江省科学院自然与生态研究所,哈尔滨150040 [2]黑龙江省林业科学院,哈尔滨150081

出　　处：《分子植物育种》2022年第24期8179-8185,共7页Molecular Plant Breeding

基　　金：黑龙江省自然科学基金项目(LH2019C077);黑龙江省科学院科学研究基金项目(KY2020ZR06);黑龙江省科学院创新基金杰青项目(CXJQ2021ZR02)共同资助;中央引导地方科技发展专项(ZY20B15)。

摘　　要：本研究旨在对3种不同生境下小叶章(Deyeuxia angustifolia)的基因组DNA进行甲基化分析。应用荧光标记甲基化敏感扩增多态性(F-MSAP)方法分析3种生境下共计57个小叶章样本的DNA甲基化程度。结果表明57份小叶章样品,采用筛选出的8对MSAP引物共扩增到1 417个位点,以Ⅳ甲基化模式为主,平均全甲基化率或位点缺失率为36.27%,Ⅱ型和Ⅲ型甲基化模式最少,平均半甲基化率和内侧甲基化率分别为17.63%、18.59%;3种生境下MSAP位点信息的Shannon多态性指数最高的是典型草甸(I=0.568 7),最低的是沼泽化草甸(I=0.557 8);在表观遗传上,典型草甸和沼泽化草甸的材料能相对的各自聚类到一组,而沼泽小叶章的材料与典型草甸和沼泽化草甸有一定交集。本研究通过F-MSAP技术对小叶章不同生境甲基化表观遗传多样性进行研究,为小叶章适应环境的表观遗传机制研究提供基础数据。The aim of this study was to analyze the DNA methylations of Deyeuxia angustifolia from three different habitats. The DNA methylaiton extent in the three habitats of Deyeuxia angustifolia using the fluorescence-labeled methylation-sensitive amplified polymorphism(F-MSAP) method. The result showed that a total of 1 417 loci were detected with a combination of 8 pairs of primers in the 57 samples. Ⅳ methylation mode was dominant,with an average full methylation rate or loci deletion rate of 36.27%. Average hemi-methylation rate and internal methylation rate were 17.63% and 18.59%, respectively. Shannon index based on methylation loci had the highest value(I=0.568 7) in the habitats of typical meadow(no ponding on the surface), the habitats of marshland meadows(seasonal ponding) had the lowest Shannon index(I=0.557 8). Samples in the habitats of typical meadow and marshland meadows were assigned to respective cluster based on methylated loci, samples in the habitats of marshes were clusted together with the other two habitas. In this study, the epigenetic diversity of Deyeuxia angustifolia in the three habitats(typical meadow, marshland meadows, marshes) was assessed through fluorescence-labeled methylation-sensitive amplification polymorphism(F-MSAP). This study provided some basic data for future studies on the epigenetic mechanisms and the environmental adaptability of the Deyeuxia angustifolia.

关 键 词：小叶章 甲基化敏感扩增多态性 DNA甲基化 表观遗传 

分 类 号：S543.9[农业科学—作物学]