NH_(4)H_(2)PO_(4)在甘蔗组培苗繁育中的应用  

Application of NH_(4)H_(2)PO_(4) in Sugarcane Tissue Culture

作　　者：张荣华[1,2] 刘昔辉[1,2] 周会[1,2] 韦金菊[1,2] 桂意云[1,2] 李海碧[1,2] 毛莲英 李松 卢曼曼[1,2] 刘俊仙[1,2] Zhang Ronghua;Liu Xihui;Zhou Hui;Wei Jinju;Gui Yiyun;Li Haibi;Mao Lianying;Li Song;Lu Manman;Liu Junxian(Sugarcane Research Center of Chinese Academy of Agricultural Sciences,Nanning,530007;Guangxi Key Laboratory of Sugarcane Genetic Improvement,Key Laboratory of Biotechnological and Genetic Improvement of Sugarcane in Guangxi(Ministry of Agriculture),Guangxi Academy of Agricultural Sciences,Nanning,530007)

机构地区：[1]中国农业科学院甘蔗研究中心,南宁530007 [2]广西壮族自治区农业科学院,农业部广西甘蔗生物技术与遗传改良重点实验室,广西甘蔗遗传改良重点实验室,南宁530007

出　　处：《分子植物育种》2022年第24期8194-8200,共7页Molecular Plant Breeding

基　　金：广西创新驱动发展专项(桂科AA17202042-9);广西农业科学院资金专项(桂农科2021YT005;桂农科2021YT014,桂农科2021YT006);中央引导地方项目(桂科ZY20198005)共同资助。

摘　　要：本研究以常规MS培养基为对照(CK),探究用磷酸二氢铵(NH_(4)H_(2)PO_(4))代替硝酸铵(NH_(4)NO_(3))对甘蔗组培苗腋芽分化、增殖、生根和生根苗丛栽成活的影响。结果表明:LS1、LS2、LS3三种培养基的甘蔗腋芽分化与对照处理相比差异不显著,且生根效果都比CK好,其中LS3培养基生根效果最好,丛栽成活率也最高,但LS3和CK相比增殖差异不显著。综上,甘蔗组织培养上用NH_(4)H_(2)PO_(4)代替NH4NO3是可行的,研究结果为甘蔗组培苗培养基的研究和应用提供理论支持。In this study,the conventional MS medium (MS) was used as control (CK).The effects of ammonium dihydrogen phosphate (NH_(4)H_(2)PO_(4)) instead of ammonium nitrate (NH_(4)NO_(3)) on axillary bud differentiation,proliferation,rooting and survival of rooting seedlings were studied.The results showed that there was no significant difference in axillary bud differentiation of LS1,LS2 and LS3 compared with CK.The rooting rates of LS1,LS2and LS3 were better than CK,among which LS3 had the highest rooting rate and survival rate of seedling cluster.There was no significant difference in tissue culture seedlings proliferation of LS3 compared with CK.The above results showed that it is feasible to replace NH_(4)H_(2)PO_(4) with NH_(4)NO_(3) in sugarcane tissue culture,and the results provide theoretical support for the research and application of sugarcane tissue culture medium.

关 键 词：甘蔗 果蔗 组织培养 NH_(4)H_(2)PO_(4) 繁育 

分 类 号：S566.1[农业科学—作物学]