Metagenomic Analysis of Mangshan Pit Viper (Protobothrops mangshanensis) Gut Microbiota Reveals Differences among Wild and Captive Individuals Linked to Hibernating Behaviors  

作　　者：Bing ZHANG Xiangyun DING Jianping JIANG Linhai LI Daode YANG 

机构地区：[1]Institute of Wildlife Conservation,Central South University of Forestry and Technology,Changsha 410004,Hunan,China [2]Qilu Normal University,Jinan 250200,Shandong,China [3]Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041,Sichuan,China [4]Department for Wildlife and Forest Plants Protetion,National Forestry and Grassland Administration,Beijing 100714,China

出　　处：《Asian Herpetological Research》2022年第4期251-268,共18页亚洲两栖爬行动物研究（英文版）

基　　金：supported by the National Natural Science Foundation of China (Grant No. 31472021);the Project for Wildlife Conservation and Management of the National Forestry and Grassland Administration of China (Grant No. 2021-HN-001);the Wildlife Conservation Project of Hunan Province (Grant No. HNYB2019-001)

摘　　要：Gut microbiota play important roles in the immunity,digestion,and energy meta bolism of their reptile hosts.Mangshan pit viper(Protobothrops mangshanensis)is a critically endangered snake species that is a Class I national protected species in China.Little is known regarding the relationship between P.mangshanensis and their gut microbial communities.In this study,the gut microbiota of wild P.mangshanensis individuals,artificially hiberna ting captive individuals,and non-hibernating captive individuals were compared across nine samples.Comparative shotgun metagenomic analysis was used to investigate the taxonomic composition,diversity,and function of P.mangshanensis gut microbial communities and assess whether their gut microbiomes were affected by their living environments and captivity conditions.The dominant phyla within P.mangshanensis gut microbial communities were Proteobacteria(65.55%),Bacteroidetes(15.97%),and Firmicutes(8.11%).Enriched functional pathways within the gut microbiota included meta bolism(54.9%),environmental information processing(9.67%),and genetic information processing(9.37%).Wild snake gut communities exhibited higher microbial diversity than the other two groups.The gut microbiomes of wild and hibernating captive snakes may be more reflective of healthy intestinal homeostasis than that in nonhibernating snakes.Specifically,non-hibernating snakes exhibited increased levelsof potentially pathogenic populations and functional specialization within gut microbial communities.Thus,different livingenvironments and captivitymethodsmay correspond to major shifts in microbiota composition,diversity,and function within P.mangshanensis.This study provides important insights to help guide the conservation of P.mangshanensis,while also carrying broad implications for our understanding of the effects of living environments and non-hibernating captivity conditions on the gut microbiota of snakes.

关 键 词：composition and diversity gene functions and pathways gutmicrobiota METAGENOMICS Protobothrops mangshanensis 

分 类 号：Q953[生物学—动物学]