马尾松松针提取物对人毛乳头细胞的保护作用及机制研究  

Protective effect and mechanism of Pinus massoniana pine needle extract in human dermal papilla cells

作　　者：王建成 邢岩 徐婷 王玥 李永强 张丽梅 Jiancheng Wang;Yan Xing;Ting Xu;Yue Wang;Yongqiang Li;Limei Zhang(Guozhen Health Research Institute,New Era Health Industry(Group)Co.,Ltd.,Beijing 102206,China)

机构地区：[1]新时代健康产业(集团)有限公司国珍健康研究院,北京102206

出　　处：《日用化学工业（中英文）》2023年第1期62-70,共9页China Surfactant Detergent & Cosmetics

摘　　要：研究了马尾松松针提取物对人毛乳头细胞(Human dermal papilla cells,HDP)的保护作用及机制。通过制备并分析不同溶剂松针提取物中主要活性成分含量;建立二氢睾酮(Dihydrotestosterone,DHT)诱导HDP细胞损伤模型,采用CCK8法检测细胞相对活力,qPCR检测细胞中血管内皮生长因子(Vascular endothelial growth factor,VEGF)、转化生长因子-β2(Transforming growth factor-β2,TGF-β2)、β-连环蛋白(β-Catenin)和蓬乱蛋白1(Dishevelled 1,DVL1)基因相对表达量,探究松针提取物对Wnt/β-Catenin信号通路的影响;最后采用UPLC-QTOF/MS分析鉴定提取物成分。结果表明,松针50%醇提物可显著提高HDP细胞相对活力,最高达88.3%(P<0.05);显著提高VEGF mRNA表达量(P<0.05),降低TGF-β2 mRNA相对表达量(P<0.01);并显著促进Wnt/β-Catenin信号中DVL1 mRNA和β-Catenin mRNA表达(P<0.05,P<0.01)。从松针50%醇提物中共鉴定出36种成分,包括黄酮类、木脂素类、生物碱和萜类等化合物。马尾松松针提取物对人毛乳头细胞具有保护作用,其机制可能与Wnt/β-Catenin信号传导途径有关。The effect and mechanism of Pinus massoniana pine needle extract on the activity of protecting human dermal papilla cells(HDP)were explored in this paper.The different extracts from pine needle were prepared with different solvents,and the content of active components was determined and analyzed by the corresponding determination method.HDP cells were induced by dihydrotestosterone(DHT)to establish androgenic alopecia cell model.CCK8 method was used to analyze the effect of pine needle extracts on the relative viability of the cells,and qPCR was adopted to investigate the effect of pine needle extract on regulating Wnt/β-Catenin signaling pathway by detecting the relative expression of VEGF,TGF-β2,β-Catenin,and DVL1 genes.UPLC-QTOF/MS was used to identify the components of the pine needle extract.The results show that the 50%alcohol extract of pine needle can significantly improve the relative activity of HDP cells(P<0.05),increase the expression of VEGF mRNA(P<0.05),decrease TGF-β2 mRNA relative expression(P<0.01),and promote the expression of DVL1 mRNA andβ-Catenin mRNA expression in the Wnt/β-Catenin signaling pathway(P<0.05,P<0.01).In addition,a total of 36 components are identified from the 50%alcohol extract of pine needle,including flavonoids,lignans,alkaloids,and terpenes.Pinus massoniana pine needle extract has a protective effect on HDP cells,and its mechanism may be related to Wnt/β-Catenin signaling pathway.

关 键 词：马尾松松针 二氢睾酮 人毛乳头细胞 雄激素性脱发 

分 类 号：TQ658[化学工程—精细化工]