不同制备方法与制备后速冻时间制备冷沉淀凝血因子的变化  

Changes of cryoprecipitate coagulation factors prepared by different preparation methods and quick-freezing time

作　　者：吴丹 施柳燕 WU Dan;SHI Liuyan(Nantong Central Blood Station,Jiangsu 226000,China)

机构地区：[1]南通市中心血站,江苏南通226000

出　　处：《中国输血杂志》2022年第10期1065-1068,共4页Chinese Journal of Blood Transfusion

摘　　要：目的 分析不同制备方法及制备后速冻时间制备冷沉淀凝血因子中纤维蛋白原(Fibrinogen, Fg)含量和Ⅷ因子含量的变化。方法 将400 mL全血的新鲜冰冻血浆(FFP)随机分为6组制备冷沉淀凝血因子,每组各20例,A1组:全自动冷沉淀制备仪法+制备后速冻时间小于1h;A2组:离心后全自动全血成分分离机制备法+制备后速冻时间小于1h;A3组:离心后手工制备法+制备后速冻时间小于1 h;B1组:全自动冷沉淀制备仪法+制备后速冻时间大于1 h;B2组:离心后全自动全血成分分离机制备法+制备后速冻时间大于1 h;B3组:离心后手工制备法+制备后速冻时间大于1 h,用全自动血凝分析仪测定Fg含量和Ⅷ因子含量,并进一步统计学分析。结果 6组Fg含量分别为(A1:245.29±27.44,A2:227.13±18.68,A3:221.11±20.95,B1:182.12±9.15,B2:163.68±15.50,B3:155.61±19.28)mg,Ⅷ因子含量分别为(A1:115.86±27.99,A2:93.79±36.29,A3:91.92±34.75,B1:83.04±18.82,B2:66.33±19.57,B3:69.34±13.26)IU。A、B两组在性别、年龄等一般情况均无统计学差异。A1组Fg含量及Ⅷ因子含量明显高于A2及A3组,有统计学差异(P<0.05)。B1组Fg含量及Ⅷ因子含量明显高于B2及B3组,有统计学差异(P<0.05)。且3种制备方法均可见制备后1h内速冻组的Fg含量及Ⅷ因子含量高于制备后大于1h速冻组,有统计学差异(P<0.05)。结论 全自动冷沉淀制备仪制备冷沉淀凝血因子后速冻时间小于1h所得冷沉淀凝血因子效率更高,质量更优。Objective To analyze the changes of fibrinogen(Fg) and Ⅷ factor levels of cryoprecipitated coagulation factors prepared by different methods and post-preparation quick-freezing time. Methods The fresh frozen plasma(FFP), prepared from 400 mL whole blood, was randomly divided into 6 groups(group A1, A2, A3,B1, B2, B3) with 20 eliquots each, to prepare cryoprecipitate coagulation factors. Group A1, B1 were prepared by automatic cryoprecipitation preparation instrument. group A2, B2 applied the instrument after centrifugation and group A3,B3 were prepared manually. The quick-freezing time after preparation in group A1-A3 and B1-B3 were different(within 1 hour vs. more than 1 hour after preparation). The automated coagulation analyzer was used to measure Fg and Ⅷ factor levels in six groups, and further statistical analysis was carried out. Results The Fg content(mg) of six groups were 245.29±27.44 in group A1, 227.13±18.68 in group A2, 221.11±20.95 in group A3, 182.12±9.15 in group B1, 163.68±15.50 in group B2, and 155.61±19.28 in group B3, respectively. The Ⅷ factor levels(IU) were of six groups were 115.86±27.99 in group A1, 93.79±36.29 in group A2, 91.92±34.75 in group A3, 83.04±18.82 in group B1, 66.33±19.57 in group B2, and 69.34±13.26 in group B3, respectively. There were no significant differences in gender or age between group As and groups Bs. The levels of Fg and Ⅷ factors in group A1 were significantly higher than those in group A2 and group A3(P<0.05). In addition, the levels of Fg and Ⅷ factors in group B1 were also obviously higher than those in group B2 and group B3(P<0.05). Further, the levels of Fg and Ⅷ factors in group As were significantly higher than those in group Bs(P<0.05). Conclusion The automatic cryoprecipitation preparation instrument plus quick-freezing within 1 hour after preparation contribute to a higher efficiency and better quality than others.

关 键 词：冷沉淀凝血因子 制备方法 制备后速冻时间 Ⅷ因子含量 纤维蛋白原含量 

分 类 号：R973.2[医药卫生—药品] R457.14[医药卫生—药学]