盐胁迫对5份无芒雀麦苗期生长和生理生化的影响及综合性评价  

Effects of salt stress on the growth,physiology,and biochemistry of five Bromus inermis varieties

作　　者：李瑞强 王玉祥 孙玉兰 张磊[1] 陈爱萍[1] LI Rui-qiang;WANG Yu-xiang;SUN Yu-lan;ZHANG Lei;CHEN Ai-ping(College of Grassland Science,Xinjiang Agricultural University,Ministry of Education Key Laboratory for Western Arid Region Grassland Resources and Ecology,Xinjiang Key Laboratory of Grassland Resources and Ecology,Urumqi 830052,China)

机构地区：[1]新疆农业大学草业学院,西部干旱荒漠区草地资源与生态教育部重点实验室,新疆草地资源与生态实验室,新疆乌鲁木齐830052

出　　处：《草业学报》2023年第1期99-111,共13页Acta Prataculturae Sinica

基　　金：财政部和农业农村部:国家现代农业产业技术体系资助。

摘　　要：为了明确不同无芒雀麦苗期耐盐能力的强弱,本研究选择5份不同株型的无芒雀麦作为供试材料。根据新疆土壤盐碱成分设置中性盐(M盐):NaCl∶Na_(2)SO_(4)∶Na_(2)CO_(3)=1∶4∶0和碱性盐(A盐):NaCl∶Na_(2)SO_(4)∶Na_(2)CO_(3)=1∶1∶8两种处理,于三叶期每盆一次性浇灌2 L电导率(EC)为20 ms·cm^(-1)的盐处理液,CK浇灌等量自来水。处理30 d后测定生长指标、叶绿素荧光特性参数、丙二醛(MDA)和渗透调节物质含量,并通过相关性分析、主成分分析、隶属函数分析对13个指标进行分析评价。结果表明:除Fv/Fm和Fv/Fo外,盐胁迫显著影响其余11个指标,且5份材料间差异显著(P<0.05);5份无芒雀麦的株高、茎粗、分蘖数、地上生物量、根冠比、MDA和脯氨酸含量在不同盐胁迫下表现出不同的适应变化;L2和L4的株高均表现出A盐处理显著高于M盐处理;A盐处理显著增加L3的地上生物量,而M盐处理与CK相比无显著差异;L1、L2和L5的MDA含量在A盐处理下的增幅明显大于M盐处理,A盐处理下L2的MDA含量明显高于其他材料;盐胁迫严重抑制了无芒雀麦的根系发育以及可溶性糖含量的积累,5份材料的地下生物量和可溶性糖含量较CK相比均出现不同程度的降低。主成分分析结果表明,渗透调节物质和生长指标可作为评价无芒雀麦耐盐能力强弱的综合指标。由耐盐评价D值得出L4耐盐能力较强,L5耐盐能力较弱。The aim of this study was to compare the salt tolerance of different Bromus inermis varieties.Seedlings of five B.inermis varieties(L1-5)were used as the experimental materials.According to the composition of soil salts in Xinjiang,two treatments were established;neutral salt(M salt)(NaCl∶Na_(2)SO_(4)∶Na_(2)CO_(3)=1∶4∶0)and alkaline salt(A salt)(NaCl∶Na_(2)SO_(4)∶Na_(2)CO_(3)=1∶1∶8).When seedlings reached the three-leaf stage,each pot was irrigated with 2 L salt treatment solution with an electrical conductivity(EC)of 20 ms·cm^(-1)at a one-time treatment.The control(CK)was irrigated with the same amount of tap water.After 30 days of treatment,the growth index,chlorophyll fluorescence characteristic parameters,malondialdehyde(MDA)content and osmoregulation substance content were measured.Then,correlation analysis,principal component analysis,and a membership function analysis were used to analyze and evaluate 13 indicators.The results showed that,other than maximum quantum efficiency(Fv/Fm)and potential quantum efficiency(Fv/Fo),the other 11 indexes were strongly affected by salt stress,and the values of the indexes differed significantly among the five Bromus varieties(P<0.05).The plant height,stem diameter,tiller number,aboveground biomass,root-crown ratio,MDA content,and proline content of the five B.inermis varieties showed different adaptive changes under different types of salt stress.The plant height of L2and L4was significantly higher in the A salt treatment than in the M salt treatment;the A salt treatment significantly increased the aboveground biomass of L3,while the M salt treatment had no significant effect compared with CK.The MDA content of L1,L2,and L5increased more under the A salt treatment than under the M salt treatment,and the MDA content of L2was higher than that in the other lines under the A salt treatment.Salt stress seriously inhibited root development and the accumulation of soluble sugars in all B.inermis varieties.The underground biomass and soluble sugar contents in t

关 键 词：无芒雀麦 盐碱胁迫 相关性分析 主成分分析 隶属函数分析 

分 类 号：S543.9[农业科学—作物学]