一种基于罗丹明6G的“关——开”型铜离子探针  

An“off-on”copper ion probe based on rhodamine 6G synthesis

作　　者：覃于鑫 陈大朔 何书平 孟倩 姚俊雄 白翠冰 魏标 苗慧 乔瑞 QIN Yuxin;CHEN Dashuo;HE Shuping;MENG Qian;YAO Junxiong;BAI Cuibing;WEI Biao;MIAO Hui;QIAO Rui(School of Chemistry and Materials Engineering,Fuyang Normal University,Fuyang Anhui 236041,China)

机构地区：[1]阜阳师范大学化学与材料工程学院,安徽阜阳236037

出　　处：《阜阳师范大学学报（自然科学版）》2022年第4期13-19,共7页Journal of Fuyang Normal University:Natural Science

基　　金：国家自然科学基金项目(21302019);安徽自然科学基金项目(1908085QB78);安徽省高校自然科学研究项目(KJ2021A0667,KJ2020A05250,KJ2021A0673);高校优秀拔尖人才培育资助项目(gxyqZD2022065);大学生创新创业项目(202110371004,S202110371019)资助。

摘　　要：通过罗丹明6G与水杨醛缩合得到荧光探针YH,并采用^(1)H NMR和^(13)C NMR表征了探针YH结构。该探针能够专一识别铜离子,通过紫外吸收光谱和荧光光谱研究YH对Cu^(2+)的识别性质,并得到其最低检出限是1.347×10^(-6)M。进一步采用红外光谱研究了两者间的作用机制。最后,通过试纸和线虫生物实验探究了YH的实际应用和生物成像的潜力。A fluorescence probe YH, which was synthesized by Rhodamine 6G and salicylaldehyde, made sure its structure by the following methods,^(1)H NMR,^(13)C NMR. It could act with Cu^(2+)sensitively and specifically, and through ultraviolet and fluorescent spectrum, it could make sure identification nature between YH and Cu^(2+), the detection limit of this probe for Cu^(2+)was1.347×10^(-6)M. The interaction mechanism between them was further studied by infrared experiment. Finally, a paper strip and nematodes experiment were carried out to study the practical application and application potential of biological imaging of YH.

关 键 词：罗丹明6G 荧光探针 Cu^(2+) 

分 类 号：O469[理学—凝聚态物理]