1,8-桉叶油素型油樟茎段组织培养研究  

Tissue culture technology of stem segment of Cinnamomumlongepaniculatum ct.1,8-eucalyptus

作　　者：肖祖飞 钟丽萍 魏希 张北红[1] 李凤[1] XIAO Zufei;ZHONG Liping;WEI Xi;ZHANG Beihong;LI Feng(Jiangxi Provincial Engineering Research Center of Seed-Breeding and Utilization of Camphor Trees,Nanchang Institute of Technology,Nanchang 330099,China)

机构地区：[1]南昌工程学院江西省樟树繁育与开发利用工程研究中心,江西南昌330099

出　　处：《南昌工程学院学报》2022年第6期29-33,101,共6页Journal of Nanchang Institute of Technology

基　　金：江西省林业局林业科技创新专项(创新专项[2019]04号);江西省重点研发计划项目(20192BBFL60012);江西省林业局樟树研究专项(创新专项[2020]07);江西省重大科技研发专项(20203ABC28W016)。

摘　　要：以1,8-桉叶油素型油樟2年生扦插苗1年生半木质化枝条为材料,对1,8-桉叶油素型油樟茎段组织培养进行研究。结果表明:5月份采集1,8-桉叶油素型油樟半木质化茎段,用质量浓度0.1%的HgCl 2消毒5 min,茎段的萌芽率最高,褐化率和污染率低;茎段萌芽最适培养基为MS+1.0 mg/L 6-BA+0.05 mg/L IBA,萌芽率为73.33%;最适增殖培养基为MS+1.0~2.0 mg/L 6-BA+0.05 mg/L IBA,增殖系数达到3.7;组培苗生根最适培养基为1/2 MS+0.5mg/L NAA,生根率达到70%。组培苗瓶内生根培养20~25 d,移入大棚内炼苗7~10 d,移栽到营养钵,移栽成活率可达80%以上。The stem tissue culture of C.longipaniculatum ct.1,8-eucalyptus was studied with annual semi-ligninized branches of C.longipaniculatum ct.1,8-eucalyptus to provide theoretical basis for rapid propagation of C.longipaniculatum nursery stock.The results show that the axillary bud germination rate of stem segments from semi-lignified stem segments of C.longipaniculatum ct.1,8-eucalyptus was the highest with 0.1%HgCl 2 disinfected for 5 min in the May,and the browning rate and pollution rate were low.The optimal medium for the germination of the stem segments of C.longipaniculatum ct.1,8-eucalyptus was MS+1.0 mg/L 6-BA+0.05 mg/L IBA,and the germination rate was 73.33%.The optimum medium for multiplication of plantlets of C.longipaniculatum ct.1,8-eucalyptus was MS+1.0~2.0 mg/L 6-BA+0.05 mg/L IBA,and the multiplication coefficient was 3.7.The suitable medium for rooting was 1/2 MS+0.5 mg/L NAA,and the rooting rate was 70.00%.After 20~25 days of rooting culture and 7 days of seedling training in plastic shed,after transplanted to a nutrition bowl,the survival rate of transplanting could reaches over 80%.

关 键 词：组织培养 1 8-桉叶油素型油樟 茎段 

分 类 号：S792.23[农业科学—林木遗传育种]