一种基于光交联明胶-聚丙烯酰胺的神经组织工程构建物  

A Neural Tissue Engineering Construct Based on Photocrosslinked Gelatin-Polyacrylamide

作　　者：安玉川 成荣 孙蕾[1,2] 桑胜波 AN Yuchuan;CHENG Rong;SUN Lei;SANG Shengbo(Shanxi Key Laboratory of Micro Nano Sensors&Artificial Intelligence Perception,Taiyuan University of Technology,Taiyuan 030024,China;Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education,Taiyuan University of Technology,Taiyuan 030024,China;Shanxi Institute of 6D Artificial Intelligence Biomedical Science,Taiyuan 030031,China)

机构地区：[1]太原理工大学微纳传感与人工智能感知山西省重点实验室,太原030024 [2]太原理工大学新型传感器与智能控制教育部重点实验室,太原030024 [3]山西省六维人工智能生物医学研究院,太原030031

出　　处：《太原理工大学学报》2023年第1期170-177,共8页Journal of Taiyuan University of Technology

基　　金：国家自然科学基金面上资助项目(51975400);国家自然科学基金重点资助项目(62031022)。

摘　　要：从改善神经组织再生材料的理化性能角度出发,选择生物相容好的光交联明胶(GelMA)和亲水聚合物聚丙烯酰胺(PAM)构建复合水凝胶支架,用于支持雪旺细胞生长。以纯GelMA作为对照,分别测试不同浓度的光交联明胶-聚丙烯酰胺复合水凝胶(GelMA-PAM)的形貌、理化性能和机械性能;观察雪旺细胞在支架上的生物相容性、增长情况以及分化程度。实验结果表明,PAM可以改善GelMA支架的机械性能,当GelMA与PAM的质量分数分别为10%和2.5%时,该支架具有良好的生物相容性,可促进雪旺神经细胞分化。因此,开发基于GelMA-PAM复合水凝胶支架可作为一种理想的神经组织工程构建物。From the perspective of improving the physicochemical properties of nerve tissue regeneration materials, biocompatible photocrosslinked gelatin(GelMA) and hydrophilic polymer polyacrylamide(PAM) were selected to construct a composite hydrogel scaffold for supporting Schwann cell growth. With pure GelMA as a control, the morphology, physicochemical properties and mechanical properties of photocrosslinked GelMA-PAM composite hydrogels with different concentrations were tested;The biocompatibility, growth, and differentiation of Schwann cells on scaffolds were observed. The experimental results show that PAM can improve the mechanical properties of GelMA scaffolds. When the mass fractions of GelMA and PAM are 10% and 2.5%, respectively, the scaffolds have good biocompatibility and can promote the differentiation of Schwann neurons. Therefore, the GelMA-PAM composite hydrogel scaffold developed in this experiment can be used as an ideal neural tissue engineering construct.

关 键 词：光交联明胶 聚丙烯酰胺 雪旺细胞 神经组织工程 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]