加味当归补血汤对糖尿病肾病大鼠肾组织miRNA-155及SOCS1的影响  

Effects of Jiawei Danggui Buxuetang on miRNA-155 and SOCS1 in renal tissue of DKD rats

作　　者：王逸凡 顾悦 申宇航 丁鑫 吕哲 王新爱 郭登洲 WANG Yi-fan;GU Yue;SHEN Yu-hang;DING Xin;LYU Zhe;WANG Xin-ai;GUO Deng-zhou(The First Affiliated Hospital of Hebei University of Chinese Medicine,Shijiazhuang 050000,China)

机构地区：[1]河北中医学院第一附属医院,河北石家庄050000

出　　处：《海南医学院学报》2023年第1期23-29,35,共8页Journal of Hainan Medical University

基　　金：政府资助临床医学优秀人才培养项目(13000022P00DE34100247);河北省中医药管理局科研计划项目(2021167);河北中医学院研究生创新能力培养资助项目(XCXZZBS2022018)。

摘　　要：目的:通过实验研究加味当归补血汤对糖尿病肾病(DKD)大鼠肾组织microRNA-155(miR-155)及细胞因子信号转导抑制因子1(SOCS1)表达水平的影响,探讨加味当归补血汤治疗DKD的作用机制。方法:选取6周龄SPF级雄性SD大鼠50只,按照随机数字表法分为正常组(CON)和建模组。建模组使用高糖高脂饲料进行为期6周的喂养后,通过链脲佐菌素(STZ)腹腔注射(35 mg/kg)建立DKD大鼠模型。建模成功后随机数字表法分为模型组(MOD)、加味当归补血汤低剂量组(DBTL)、加味当归补血汤中剂量组(DBTM)、加味当归补血汤高剂量组(DBTH)、厄贝沙坦组(IRB),各给药组分别灌胃给药20周,CON组与MOD组给予同等体积生理盐水灌胃。20周结束后收集尿标本,全自动分析仪分析大鼠尿微量白蛋白与肌酐比值(UACR),取材后采用苏木精-伊红(HE)染色法、马松(Masson)三色染色法、过碘酸六胺银(PASM)染色法分别观察各组大鼠肾组织形态病理学变化;Real-time PCR检测DKD大鼠肾组织miR-155、SOCS1 mRNA的表达情况,蛋白免疫印迹法(Western Blot)测定DKD大鼠肾脏标本中SOCS1蛋白表达情况。结果:与CON组相比,MOD组大鼠UACR值明显升高(P<0.01);MIR-155 mRNA表达量明显升高(P<0.01);SOCS1 mRNA表达量明显降低(P<0.01);与MOD组相比,IRB组及DBT各剂量组UACR均显著降低(P<0.01);DBTM组、DBTH组及IRB组SOCS1表达量均有明显升高(P<0.01);DBTL组差异无统计学意义(P>0.05);各给药组MIR-155表达量显著降低(P<0.05,P<0.01);DBTM组、DBTH组及IRB组中SOCS1表达显著提高(P<0.01)。结论:加味当归补血汤可能通过降低高糖状态下miR-155的表达,提高肾组织内SOCS1的表达,减轻肾脏损害,发挥其肾保护作用。Objective:To observe the effect of Jiawei Danggui Buxuetang on the expression of microRNA-155(miR-155)and cytokine signal transduction inhibitor 1(SOCS1)in renal tissue of rats with diabetic nephropathy(DKD),and to explore the mechanism of Jiawei Danggui Buxuetang in the treatment of DKD.Methods:Firty 6-week-old SPF male SD rats were randomly divided into normal group(CON)and model group.The model group was fed with high-sugar and high-fat diet for 6 weeks,and the DKD rat model was established by intraperitoneal injection of streptozotocin(STZ)(35 mg/kg).Then the rats were randomly divided into model group(MOD),low dose group(DBTL),medium dose group(DBTM),high dose group(DBTH)and irbesartan group(IRB).Each group was given intragastric administration for 20 weeks,during which CON group and MOD group were given the same amount of normal saline.Urine samples were collected after administration,and the ratio of urinary Microalbumin to urinary creatinine(UACR)was analyzed by automatic analyzer.The renal changes in each group were observed by hematoxylin-eosin(HE)staining,Masson pine(Masson)trichromatic staining and PASM staining.The expression of miR-155 and SOCS1mRNA in renal tissue of DKD rats was detected by Real-time PCR,and the expression of SOCS1 was detected by Western blotting.Results:By comparison with CON group,UACR value,MIR-155 mRNA expression and SOCS1 mRNA expression in MOD group increased significantly,while SOCS1 mRNA expression decreased significantly compared with MOD group,UACR decreased significantly in IRB group and DBT group,SOCS1 expression increased significantly in DBTM group,DBTH group and IRB group.The expression of MIR-155 decreased significantly in all treatment groups(P<0.05),and the expression of SOCS1 in DBTM group,DBTH group and IRB group increased significantly(P<0.05).Conclusion:Jiawei Danggui Buxuetang can reduce the expression of miR-155 in high glucose and increase the expression of SOCS1 in renal tissue,so as to significantly reduce renal damage and play a role in renal protectio

关 键 词：加味当归补血汤 糖尿病肾病 厄贝沙坦 microRNA-155 细胞因子信号转导抑制因子1 

分 类 号：R285.5[医药卫生—中药学]