circRNA CPT1A在2型糖尿病患者外周血的差异表达  

Differential expression of circRNA CPT1A in peripheral blood of patients with type 2 diabetes mellitus

作　　者：王娟[1] 常向云[1] 朱余蓉[1] 巴涛 WANG Juan;CHANG Xiang-yun;ZHU Yu-rong;BA Tao(Department of Endocrinology and Metabolism,the First Affiliated Hospital of Shihezi University School of Medicine,Shihezi 832008,China)

机构地区：[1]新疆石河子大学医学院第一附属医院内分泌代谢科,新疆石河子832000

出　　处：《海南医学院学报》2023年第1期30-35,共6页Journal of Hainan Medical University

基　　金：国家自然科学基金资助项目(81860149)。

摘　　要：目的:通过观察circRNA CPT1A在2型糖尿病患者外周血中的相对表达水平,探讨circRNA CPT1A在2型糖尿病中的临床价值。方法:收集石河子大学医学院第一附属医院就诊的20例2型糖尿病患者及20例健康体检者的外周血以及一般资料,完善糖耐量试验,检测糖尿病及健康对照组外周血的相关生化指标,通过qRT-PCR技术检测circRNA CPT1A的相对表达水平;采用ROC曲线评价circRNA CPT1A在T2DM中的诊断价值;采用相关性分析探讨目标circRNA与糖脂代谢指标之间的相关性;采用Logistic回归模型分析circRNA CPT1A对T2DM患病风险的影响。结果:2型糖尿病组circRNA CPT1A相对表达水平均高于健康对照组(P<0.001)。ROC曲线显示circRNA CPT1A在2型糖尿病中具有一定的诊断价值。相关性分析中显示circRNA CPT1A的表达水平与空腹血糖、糖负荷后2 h血糖、HOMA-IR、HOMA-β、甘油三酯存在着相关关系(P<0.05)。Logistic回归分析显示circRNA CPT1A相对表达水平可升高2型糖尿病的患病风险(P<0.05)。结论:circRNA CPT1A在2型糖尿病患者外周血中的表达水平显著升高,提示它们有可能成为2型糖尿病的潜在诊断标志物。Objective:To investigate the clinical value of circRNA CPT1A in type 2 diabetes mellitus(T2DM)by observing the relative expression levels of circRNA CPT1A in the peripheral blood of type 2 diabetic patients.Methods:Peripheral blood,as well as general data were collected from 20 type 2 diabetic patients and 20 healthy check-ups attending the First Affiliated Hospital of Shihezi University School of Medicine,and a glucose tolerance test was perfected to detect relevant biochemical indexes in peripheral blood of diabetic and healthy control groups,and the relative expression level of circRNA CPT1A was detected by qRT-PCR technique;ROC curve was used to evaluate the diagnostic value of circRNA CPT1A in T2DM;correlation analysis was used to explore the correlation between target circRNA and glucolipid metabolic indexes;logistic regression model was used to analyze the effect of circRNA CPT1A on the risk of T2DM.Results:The relative expression levels of circRNA CPT1A were higher in the T2DM group than in the healthy control group(P<0.001).ROC curves showed that circRNA CPT1A has a diagnostic value in T2DM.The correlation analysis showed that the expression level of circRNA CPT1A correlated with fasting glucose,2 h post-glucose load,HOMA-IR,HOMA-β,and triglycerides(P<0.05).logistic regression analysis showed that the relative expression level of circRNA CPT1A increased the risk of T2DM(P<0.05).Conclusion:The expression levels of circRNA CPT1A were significantly elevated in the peripheral blood of T2DM patients,suggesting that they may be a potential diagnostic marker for T2DM.

关 键 词：circRNA 糖尿病 生物标志物 

分 类 号：R587.1[医药卫生—内分泌]