2015—2020年黑龙江省脊髓灰质炎实验室细胞敏感性监测与评价  

Monitoring and evaluation of cell sensitivity in poliomyelitis laboratory in Heilongjiang Province from 2015 to 2020

作　　者：王晗[1] 宋婧[1] 吕珀[1] 孙兆丹[1] 宋长江[1] 韩宇[2] WANG Han;SONG Jing;Lü Po;SUN Zhao-dan;SONG Chang-jiang;HAN Yu(Heilongjiang Prouincial Center for Disease Control and Pretent ion,Harbin,Heilongjiang 150030,China;不详)

机构地区：[1]黑龙江省疾病预防控制中心,黑龙江哈尔滨150030 [2]内蒙古通辽市疾病预防控制中心

出　　处：《中国公共卫生管理》2022年第5期673-676,共4页Chinese Journal of Public Health Management

摘　　要：目的评价2015—2020年黑龙江省脊髓灰质炎(以下简称脊灰)实验室细胞系质量控制情况。方法采用巢氏聚合酶链式反应(PCR)方法对细胞支原体进行检测,采用96孔微量板病毒滴定法对脊灰病毒的敏感性进行检测,并对检测结果进行分析。结果黑龙江省脊灰实验室在2015—2020年使用的细胞系支原体检测均为阴性,3个型别的脊灰病毒敏感性分别为:Sabin 1在人横纹肌肉瘤细胞系(rhabdomyosarcoma,RD)细胞上滴度为108.23±0.23,在表达人脊灰病毒的小鼠肺细胞系(Mouse Lung Cells that Express Receptors for HumanPolioviruses,L20B)细胞上滴度为10^(6.97±0.37);SabinⅡ在RD细胞上滴度为10^(8.27±0.47),在L20B细胞上滴度为10^(6.92±0.17);SabinⅢ在RD细胞上滴度为10^(8.10±0.40),在L20B细胞上滴度为10^(6.60±0.40),均在有效范围内波动。结论2015—2020年,黑龙江省脊灰实验室细胞质量状况良好,未出现细胞支原体污染,并且RD细胞、L20B细胞对脊灰病毒及肠道病毒的敏感性均在正常范围内波动。支原体检测和细胞敏感性监测作为质量控制的重要指标,保证了实验室检测脊灰病毒的敏感性。ObjectiveTo evaluate the quality control of cell lines in poliomyelitis laboratory in Heilongjiang Province from 2015 to 2020.MethodsNested polymerase chain reaction(PCR)was used to detect mycoplasma,and the sensitivity of cells to poliovirus was detected by 96 microcells pate culture and titration method.ResultsThe detection of mycoplasma in cell lines used by Heilongjiang polio laboratory from 2015 to 2020 was negative.3 serotypes of poliovirus sensitivity were that SabinⅠtiter on RD cells was10^(8.27±0.47),the titer on L20B cells was10^(6.97±0.37),SabinⅡtiter on RD cell line was 10^(8.27±0.47),the titer on L20B cell line was10^(6.92±0.17),SabinⅢtiter on RD cell line was 10^(8.10±0.40),the titer on L20B cell line was 10^(6.60±0.40),and all fluctuated with in the effective range.ConclusionFrom 2015 to 2020,the cellquality of polio laboratory in Heilongjiang Province is good,with mycoplasma contamination.The sensitivity of L20B and RD cell lines used in the laboratory to poliovirus is within the normal range.As the important indicators of quality control,mycoplasma detection and cell sensitivity monitoring ensures the sensitivity of laboratory detection of poliovirus.

关 键 词：脊髓灰质炎 支原体检测 细胞敏感性 质量控制 

分 类 号：R446[医药卫生—诊断学]