一种新型微流控血小板功能分析技术的性能评价  

Performance Evaluation of a New Microfluidic Platelet Function Test Platform

作　　者：吴悠 肖文海 丁玲 陈丹 邓素容[1] 李远[1] WU You;XIAO Wen-Hai;DING Ling;CHEN Dan;DENG Su-Rong;LI Yuan(Central Laboratory,Yongchuan Hospital of Chongqing Medical University,Chongqing 402160,China;Department of Mammary G land,Dazu Maternal and Child Health Hospital,Chongqing 402160,China;Sub-center of Chongqing Blood Center,Chongqing 402160,China)

机构地区：[1]重庆医科大学附属永川医院中心实验室,重庆402160 [2]重庆市大足区妇幼保健院乳腺科,重庆402160 [3]重庆市血液中心永川分中心,重庆402160

出　　处：《中国实验血液学杂志》2022年第6期1893-1901,共9页Journal of Experimental Hematology

基　　金：国家自然科学基金计划资助项目(11702047);重庆市社会事业与民生保障科技创新专项(cstc2017shmsA130009);重庆市医学科研计划项目(2017MSXM079);重庆市大足区科技计划项目(DZKJ2019ACC1028)。

摘　　要：目的:对一种新型微流控血小板功能分析技术的检测性能进行评价。方法:以课题组前期搭建的微流控血小板功能检测平台为评价对象,分析流动剪切率和样品保存时间对血小板功能检测结果的影响;评价微流控血小板功能检测平台的批间变异性;采集24名健康志愿者外周静脉血,在存在20μmol/L乙酰水杨酸(COX-1抑制剂)或50μmol/L 5-磷酸-2-甲基硫代腺苷酸(P2Y12受体抑制剂)的情况下用微流控血小板功能分析平台和光透射浊度法检测血小板功能,通过受试者工作特征曲线分析两种方法检测乙酰水杨酸和5-磷酸-2-甲基硫代腺苷酸抑制血小板功能的诊断性能。结果:在1500/s流动条件下,微流控血小板功能分析技术可动态监测血小板黏附聚集行为并量化血小板功能;血小板聚集功能随流动剪切率增大而增强;样品室温放置5 h内不影响血小板功能检测结果;微流控血小板功能分析技术的批间变异性变异系数<15%;受试者工作特征曲线下面积显示,本平台对乙酰水杨酸和5-磷酸-2-甲基硫代腺苷酸抑制血小板功能的鉴别具有良好诊断性能。结论:微流控血小板功能分析技术对血小板功能检测具有良好的分析性能,未来可发展为一种适宜临床的新型血小板功能检测装置。Objective:To evaluate the performance of a microfluidic platelet function test platform(MPFTP)previously established by our research group.Methods:The effects of flow shear rate and storage time on platelet function test were analyzed taking the MPFTP as the object.The intra-assay variability of the MPFTP was evaluated.The function of platelet in peripheral venous blood from 24 healthy volunteers was assessed using the MPFTP and light transmission turbidity,either in the presence of 20μmol/L acetylsalicylic acid(AS,an inhibitor of cyclooxygenase 1)or 50μmol/L 5-phospho-2-methylthioadenosine(2-MeSAMP,a P2 Y12receptor inhibitor).The diagnostic performance of both methods in assaying platelet function inhibition by AS and 2-MeSAMP was analyzed by using receiver operating characteristic(ROC)curve.Results:Under the flow shear rate of 1500 s-1,our MPFTP could dynamically monitor platelet adhesion and aggregation,as well as quantify platelet function.Platelet aggregation increased with the increase of flow shear rate,while sample storage at room temperature for up to 5 h did not affect results of platelet function test.The intra-assay variability coefficient of variation of the MPFTP was<15%.The area under the curve of ROC showed that this platform had good diagnostic performance in the identification of platelet function inhibition by AS and 2-MeSAMP.Conclusion:This MPFTP shows good analytical performance for the assay of platelet function and can be developed into a new clinical platelet function test device in the future.

关 键 词：血小板 微流控 抗血小板药物 性能评价 

分 类 号：R331[医药卫生—人体生理学]