多能干细胞在体外心脏模型构建研究中的应用  

Application of pluripotent stem cells in establishing heart model in vitro

作　　者：高原 盛伟 黄国英 Yuan Gao;Wei Sheng;Guoying Huang(Pediatric Heart Center,Children′s Hospital of Fudan University,Shanghai 201102,China;Shanghai Key Laboratory of Birth Defects,Shanghai 201102,China;Research Unit of Early Intervention of Genetically Related Childhood Cardiovascular Diseases,Chinese Academy of Medical Sciences,Shanghai 201102,China)

机构地区：[1]复旦大学附属儿科医院心血管中心,上海201102 [2]上海市出生缺陷防治重点实验室,上海201102 [3]中国医学科学院小儿遗传相关性心血管疾病早期防控创新单元,上海201102

出　　处：《中华细胞与干细胞杂志（电子版）》2022年第5期314-318,共5页Chinese Journal of Cell and Stem Cell（Electronic Edition）

基　　金：国家重点研发计划项目(2021YFC2701000,2016YFC1000500);中国医学科学院医学与健康科技创新工程项目(2019-I2M-5-002);国家自然科学基金(81873482);上海市科学技术委员会"科技创新行动计划"基础研究领域项目(20JC1418300)。

摘　　要：传统二维细胞模型和模式生物因其自身局限性并不完全适用于心血管疾病的基础研究。多能干细胞(PSCs),包括胚胎干细胞(ESCs)和诱导PSCs (iPSCs),是一类具有自我增殖能力和多能性的细胞,可分化形成多种细胞。近年来,越来越多的研究选择利用PSCs构建体外心脏模型。目前已可诱导PSCs分化获得多种心脏谱系细胞,也可结合组织工程技术获得组织工程心脏以及在三维培养系统中获得自发形成的类器官模型。利用PSCs获得的体外心脏模型在一定程度上复现了人类心脏的自然发育过程,相比于传统模型更贴合体内心脏的生物学特性和功能,展现出其良好的应用前景。Traditional two-dimensional cell models and model organisms are not fully applicable to the basic research of cardiovascular diseases due to their own limitations. Pluripotent stem cells (PSCs) , including embryonic stem cells (ESCs) and induced PSCs (iPSCs) , are characterized by the properties of self-renewal and potency. In recent years, more and more studies have constructed in vitro cardiac models with PSCs. At present, PSCs can be induced to differentiate into a variety of cardiac lineage cells, tissue engineered hearts can be obtained by combining tissue engineering techniques, and spontaneous organoid models can be obtained in 3D culture systems. The in vitro heart model obtained by PSCs can partially repaint the natural development process of human heart, and it is more similar to the biological characteristics and functions of the heart in vivo than the traditional model, showing good application prospects.

关 键 词：体外模型 多能干细胞 心脏组织 类器官 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]