飞蝗雌成虫脂肪体G蛋白偶联受体鉴定及其在卵发育中的功能  

Identification of G protein-coupled receptors in the fat body of the female adults of Locusta migratoria(Orthoptera:Acrididae)and their functions in egg development

作　　者：贠佳琦 杨洁冰 许慧晶 郑洪远 周树堂 YUN Jia-Qi;YANG Jie-Bing;XU Hui-Jing;ZHENG Hong-Yuan;ZHOU Shu-Tang(State Key Laboratory of Cotton Biology,School of Life Sciences,Henan University,Kaifeng,Henan 475004,China)

机构地区：[1]河南大学生命科学学院,棉花生物学国家重点实验室,河南开封475004

出　　处：《昆虫学报》2022年第12期1571-1581,共11页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(U1804232,U1904103);河南省重大科技专项项目(201300311300)。

摘　　要：【目的】本研究旨在鉴定飞蝗Locusta migratoria雌成虫脂肪体中表达的G蛋白偶联受体(G protein-coupled receptor,GPCR)基因,揭示其在飞蝗卵发育和卵巢生长中的功能。【方法】基于前期获得的第1个促性腺周期内飞蝗雌成虫脂肪体转录组数据,鉴定和聚类分析GPCR基因;qRT-PCR检测GPCR基因在卵黄发生期飞蝗雌成虫脑、胸腹神经节、脂肪体、卵巢和中肠的组织特异性表达模式;通过RNAi实验分析GPCR基因在卵发育和卵巢生长中的功能。【结果】在飞蝗雌成虫脂肪体转录组中新鉴定到了29个GPCR基因。qRT-PCR分析显示,PTH/PTHrPR1和MSR分别在飞蝗雌成虫脂肪体和胸腹神经节中显著高表达,Mthl4,Mthl6和GPR119L在中肠中显著高表达,Octβ1R,CrzR,CCAPR,LGR2,mGluR和GABABR在脑中的表达水平显著高于在其他组织中的。RNAi筛选发现敲降5个GPCR基因CCAPR,PTH/PTHrPR1,ADGRL3,Mthl 15和DHR的表达显著抑制飞蝗初级卵母细胞发育和卵巢生长。【结论】本研究结果有助于揭示昆虫高繁殖力的调控网络,并发掘新型害虫防治靶标。【Aim】This study aims to identify the G protein-coupled receptor(GPCR)genes expressed in the fat body of the female adults of Locusta migratoria,and uncover their functions in egg development and ovarian growth of L.migratoria.【Methods】Based on the previously obtained transcriptome data of the fat body of the female adults of L.migratoria during the first gonadotropic cycle,identification and cluster analysis of GPCR genes were performed.qRT-PCR was employed to measure the tissue-specific expression patterns of GPCR genes in the brain,thoracic and abdominal ganglia,fat body,ovary and midgut of vitellogenic female adults of L.migratoria.RNAi experiments were carried out to analyze the functions of GPCR genes in egg development and ovarian growth.【Results】A total of 29 GPCR genes were newly identified from the fat body transcriptome of female adults of L.migratoria.qRT-PCR results demonstrated that PTH/PTHrPR1 and MSR were significantly highly expressed in the fat body and thoracic and abdominal ganglia of female adults of L.migratoria,respectively,while Mthl4,Mthl6 and GPR119L were significantly highly expressed in the midgut,and the expression levels of Octβ1R,CrzR,CCAPR,LGR2,mGluR and GABABR in the brain were significantly higher than those in the other tissues.RNAi screening revealed that knockdown of five GPCR genes,CCAPR,PTH/PTHrPR1,ADGRL3,Mthl15 and DHR,significantly inhibited the primary oocyte development and ovarian growth of L.migratoria.【Conclusion】Findings in the present study are helpful for unveiling the regulatory networks of powerful fecundity of insects and exploring novel targets for insect pest control.

关 键 词：飞蝗 G蛋白偶联受体 转录组 RNA干扰 生殖力调控 

分 类 号：Q965[生物学—昆虫学]