马尾松miR156a基因表达及其耐低磷功能分析  

Gene Expression of miR156a and Analysis of Its Tolerance to Low Phosphorus in Masson Pine

作　　者：王聪慧 范付华 覃慧娟[1,2] 尚先文 周紫晶 丁贵杰 谭健晖 Wang Conghui;Fan Fuhua;Qin Huijuan;Shang Xianwen;Zhou Zijing;Ding Guijie;Tan Jianhui(Key Laboratory of Plateau and Mountain Forest Cultivation,Guizhou Forest and Environmental Research Center,Guizhou University,Guiyang,550025;College of Forestry,Guizhou University,Guiyang,550025;Guangxi Forestry Research Institute,Nanning,530002)

机构地区：[1]贵州大学,贵州省森林资源与环境研究中心,贵州省高原山地林木培育重点实验室,贵阳550025 [2]贵州大学林学院,贵阳550025 [3]广西壮族自治区林业科学研究院,南宁530002

出　　处：《分子植物育种》2022年第21期7053-7059,共7页Molecular Plant Breeding

基　　金：国家重点研发计划项目(2017YFD0600302);国家自然科学基金项目(31660185);广西创新驱动发展专项(桂科AA17204087-4);贵州省科技计划项目(黔科合平台人才[2017]5788)共同资助。

摘　　要：马尾松(Pinus massoniana)是中国主要的针叶用材树种,具有速生、丰产、适应性强、经济价值高等优良特性。miR156家族是植物中含量丰富且高度保守的一类microRNA(miRNA),在调控植物叶片的形成、开花时间、侧根发育以及非生物胁迫中具有重要作用。为探究miR156a在马尾松幼苗响应低磷胁迫过程中的功能,比较了不同物种的miR156a的成熟序列,发现mi R156a在不同物种中序列的保守性高。对马尾松幼苗进行低磷胁迫,并分析miR156a在马尾松幼苗的时空表达模式;将Pma-miR156a转化至烟草植株并进行磷胁迫处理,观察转基因烟草地下部分根系的结构变化。结果表明,miR156a在马尾松幼苗地上部分的表达量要大于地下部分,并在磷胁迫48 d时被显著诱导表达,磷胁迫处理后的转基因植株的根系较野生型更为发达。综上所述,miR156a主要在马尾松幼苗的地上部分表达,推测其可能通过远距运输至地下部分来调节植物根系结构的变化进而响应磷胁迫,本研究为揭示马尾松耐低磷机制以及抗性种质的选育提供了一定的理论参考。Masson pine(Pinus massoniana)is a main coniferous timber species in China,which has excellent characteristics such as fast growth,high yield,strong adaptability,and high economic value.The miR156 family is an abundant and highly conserved class of microRNAs(miRNAs)in plants.It plays an important role in regulating plant leaf formation,flowering time,lateral root development,and abiotic stress.Comparing the mature sequences of Pma-mi R156a from different species revealed that miR156a is highly conserved in different species.In order to explore the function of Pma-miR156a,the temporal and spatial expression pattern pattern of miR156a were analyzed in masson pine seedlings treated with low phosphorus;Pma-mi R156a was transformed into tobacco and subjected to phosphorus stress to observe the root structural changes of transgenic tobacco.The results showed that the expression level of miR156a in the aerial part of masson pine seedlings was greater than that in the underground part,and was significantly induced at 48 d of low phosphorus stress.The root system of the transgenic plants treated with phosphorus stress was more developed than that of the wild type.In summary,miR156a is mainly expressed in the above-ground part of masson pine seedlings,and it is speculated that it may be transported to the under-ground part to regulate the changes of root structure and thus adapt to phosphorus stress,this study provides a certain theoretical reference for revealing the low-phosphorus tolerance mechanism of masson pine and breeding resistant germplasm.

关 键 词：马尾松 miR156a 低磷胁迫 组织表达模式 根系结构 

分 类 号：S791.248[农业科学—林木遗传育种]