Cullin家族通过UPS途径响应小麦生长发育及非生物胁迫进程  

Cullin Family Members Respond to Wheat Growth,Development and Abiotic Stresses via Ubiquitin-proteasome System

作　　者：赵梦伊 魏春茹 范润侨 杨一鸣 杨近炳 孟妍君 赵伟全[2] 于秀梅 Zhao Mengyi;Wei Chunru;Fan Runqiao;Yang Yiming;Yang Jinbing;Meng Yanjun;Zhao Weiquan;Yu Xiumei(Hebei Key Laboratory of Plant Physiology and Molecular Pathology,College of Life Sciences,Hebei Agricultural University,Baoding,071001;Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province,College of Plant Protection,Hebei Agricultural University,Baoding,071001)

机构地区：[1]河北农业大学生命科学学院河北省植物生理与分子病理学重点实验室,保定071001 [2]河北农业大学植物保护学院河北省农作物病虫害生物防治技术创新中心,保定071001

出　　处：《分子植物育种》2022年第21期7071-7081,共11页Molecular Plant Breeding

基　　金：河北省自然科学基金项目(C2020204050);河北省高等学校科学技术研究项目(ZD2019086);河北省研究生创新项目(CXZZBS2021046)共同资助。

摘　　要：Cullin作为泛素-蛋白酶体系统(ubiquitin-proteasome system,UPS)中多亚基E3泛素连接酶的骨架蛋白,与泛素结合酶E2相互作用,将泛素分子连接到待降解底物上,使底物携带泛素链标记,进而被26S蛋白酶体识别并降解。Cullin家族成员在植物体内发挥着重要作用,但其在小麦中的研究尚少。因此,本研究对小麦中Cullin家族基因展开了系统的分析。自小麦中鉴定了39个Cullin蛋白,由25个基因编码。大多数小麦Cullin蛋白分子量大于80 kD,偏酸性,于细胞核中发挥作用;基于结构域组成差异将小麦Cullin蛋白分为Cullin、Cullin+Cullin-Nedd8、Cullin+Cullin+Cullin-Nedd8及Cullin+ANAPC2四大类;进化关系分析表明,该家族蛋白包括四个亚家族Cullin 1~Cullin 4,结构域在该家族蛋白进化过程中起主要作用;68%的Cullin基因分布在小麦B基因组上;互作蛋白预测结果显示,小麦中Cullin蛋白大部分与UPS途径其他成员相互作用;表达模式分析表明,Cullin家族中大部分基因呈现组织专化性表达,并响应热和干旱的胁迫过程。由此可见,Cullin作为UPS途径主要成员而在小麦生长、发育及应对非生物胁迫中发挥着重要的作用,本研究结果从基因组水平分析和鉴定了小麦Cullin家族成员,为深入解析小麦中Cullin家族蛋白的生物学功能及作用网络提供了重要参考。As the scaffold protein of multi subunit E3 ligases in ubiquitin-proteasome pathway(UPS),Cullin proteins interact with ubiquitin conjugating enzyme E2,thereby connect ubiquitin to the substrate to be degraded,then the substrates labeled by ubiquitin chain are recognized and degraded by the 26S proteasome.The Cullin family members play many important roles in plants,but less is known about the Cullin family in wheat.Based on this situation,the present study carried out a genome wide analysis for wheat Cullin family.Thirty-nine Cullin proteins encoded by 25 genes were identified in the wheat.Most of them were acidic proteins with Molecular Weight of over 80 kD,and played roles in nucleus;Wheat Cullin proteins were classified into 4 types of Cullin,Cullin+Cullin-Nedd8,Cullin+Cullin+Cullin-Nedd8 and Cullin+ANAPC2 based on their different domain structure;Phylogenetic analysis showed that the family included four clades Cullin 1~Cullin 4,moreover,domains played an important role in the evolution of Cullin family;68%Cullin genes were distributed on wheat B genome;Prediction of the partner protein indicated that the large amount of wheat Cullin proteins interacted with other components of UPS;expression patterns indicated that most of the Cullin genes exhibited tissue specific expression and responded to heat and/or drought stresses.Cullin proteins played active roles in wheat growth,development and response to abiotic stresses via UPS.The aforementioned results analyzed and identified the wheat Cullin family in genome wide,and provide an important reference for dissecting their biological functions and protein-protein interaction network in wheat.

关 键 词：小麦Cullin家族 基因组鉴定 进化分析 染色体分布 蛋白互作 

分 类 号：S512.1[农业科学—作物学]