针刺疗法对衰老大鼠腰肌组织形态学及细胞凋亡的影响  

Effect of Acupuncture on Histomorphology and Apoptosis of Lumbar Muscle of Aging Rats

作　　者：林建平 郭明玲 金京 王浩 王诗忠 陈少清[2] LIN Jianping;GUO Mingling;JIN Jing;WANG Hao;WANG Shizhong;CHEN Shaoqing(The School of Health,Fujian Medical University,Fuzhou 350122,China;College of Rehabilitation Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]福建医科大学健康学院,福州350122 [2]福建中医药大学康复医学院,福州350122

出　　处：《中国中医基础医学杂志》2022年第12期1975-1979,1985,共6页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金项目(81973924)-外泌体内miR-146a介导Akt/NF-κB通路调节在针刺委中穴防治腰肌衰老中的机制研究;国家自然科学基金青年基金项目(81904317)-基于内质网应激-自噬反应研究“脾主肌肉”理论下推拿脾经治疗骨骼肌损伤的作用机制;福建省自然科学基金项目(2019J01360)-基于外泌体miR-146a介导Akt/NF-κB通路探讨针刺委中穴治疗腰痛的作用机制。

摘　　要：目的 观察针刺疗法对衰老大鼠腰椎骨骼肌质量、组织形态、超微结构及细胞凋亡的影响,探讨针刺延缓腰部骨骼肌衰老(lumbar muscle aging, LMA)的可能机制。方法 50只sprague-dawley(SD)雄性大鼠随机分为空白组、模型组、针刺穴位组、针刺非穴组、阳性药物组每组各10只,除空白组外其余4组均颈背部皮下注射2.5%D-半乳糖(D-Galactose, D-gal)制备LMA大鼠模型,空白组皮下注射等量的0.9%氯化钠溶液,每日1次,连续8周。自造模第29天起进行干预,针刺穴位组取双侧“委中”(BL40)和“肾俞”(BL23),针刺非穴组取大鼠双侧“非经非穴位”(“委中”内侧旁开0.5 cm、“肾俞”与“志室”(BL52)中点),阳性药物组采用混合氨基酸灌胃干预。测量体质量增长率、肌肉衰减征指数(sarcopenia index, SI)值,采用HE染色、透射电镜观察肌肉组织形态和超微结构,Tunel法检测肌细胞凋亡。结果 与空白组比较,模型组大鼠体质量增长率和SI值均下降(P<0.05或P<0.01),HE染色显示模型组肌细胞间隙增大出现不同程度的萎缩;超微结构观察到模型组肌浆网数量减少出现不同程度的肿胀、扩张,模型组肌细胞凋亡率升高(P<0.01)。与模型组比较,针刺穴位组、阳性药物组大鼠体质量增长率和SI值升高(P<0.05),肌纤维圆形化程度减少,细胞间隙减少,萎缩程度减轻;肌浆网形态较为正常,偶见局部轻度肿胀、扩张且肌细胞凋亡率下降(P<0.01)。结论 针刺“委中”“肾俞”可改善大鼠腰骨骼肌衰老,可能是通过减少细胞凋亡、促进肌浆网功能而发挥作用。Objective: To observe the effect of acupuncture on skeletal muscle mass, tissue morphology, ultrastructure and apoptosis of aging rats, and to study the mechanism of acupuncture on lumbar muscle aging(LMA). Methods: Fifty male sprague-dawley(SD) rats were randomly divided into normal group(n=10), model group(n=10), acupuncture point group(n=10), acupuncture non-acupoint group(n=10), and positive drug group(n=10). Except for the normal group, rats in the other 4 groups were injected subcutaneously with 12.5% D-gal on the back of the neck to establish LMA. The normal group was injected with the same amount of 0.9% sodium chloride solution, once a day, continuously for 8 weeks. From the 29th day of model establishment, the acupuncture point group received acupuncture at bilateral acupoint “Weizhong(BL40)” and “Shenshu(BL23)”. The acupuncture non-acupoint group selected bilateral "non-meridian non-acupoints" [0.5 cm lateral to the inner side of acupoint "Weizhong" and the midpoint of acupoint “Shenshu” and “Zhishi(BL52)”]. Rats in positive drug group was given mixed amino acid by gavage. The weight growth rate and wet mass ratio(SI) of bilateral erector spinae muscles were measured. Histopathological and ultrastructural changes of muscle were observed by HE staining and transmission electron microscopy. Tunel assay was used to detect the apoptosis of muscle cells. Results: Compared with the normal group, the weight growth rate and SI value decreased significantly(P<0.01) in the model group. HE staining showed that the muscle cell space increased, and atrophy of different degrees appeared in the model group. Ultrastructurally, the number of sarcoplasmic reticulum was decreased, and swelling and expansion of different degrees appeared in the model group. The apoptosis rate was significantly increased(P<0.001) in the model group. Compared with the model group, the acupuncture point group and the positive drug group showed increased weight growth rate and SI value(P<0.05), reduced muscle fibers circulariz

关 键 词：针刺 “委中” “肾俞” 肌肉衰减征 细胞凋亡 

分 类 号：R245.31[医药卫生—针灸推拿学]