寻常型银屑病皮损中CXCL8、miR-150表达与病情严重程度相关  

Correlation between the expressions of CXCL8 and miR-150 in the skin lesions and the severity of psoriasis vulgaris

作　　者：马卉[1] 毕晓东[1] MA Hui;BI Xiaodong(Department of Dermatology,Nanyang First People's Hospital,Nanyang 473001,China)

机构地区：[1]南阳市第一人民医院皮肤科,河南南阳473001

出　　处：《基础医学与临床》2023年第2期293-297,共5页Basic and Clinical Medicine

基　　金：河南省医学科技攻关计划项目(LHGJ20191499)。

摘　　要：目的探讨寻常型银屑病患者皮损组织中CXC趋化因子配体8(CXCL8)、miR-150表达与病情严重程度的关系。方法选取2020年1月至2021年5月在南阳市第一人民医院接受治疗的80例寻常型银屑病患者皮损组织作为研究对象,并选其皮损旁正常组织作为对照,用银屑病皮损面积和严重程度指数(PASI)对银屑病患者病情进行分级,用免疫组化法检测CXCL8蛋白的表达,用RT-qPCR检测miR-150表达,用Western blot检测CXCL8蛋白表达,进一步分析CXCL8蛋白、miR-150表达水平与病情严重程度的相关性。结果寻常型银屑病患者皮损组织中CXCL8 mRNA水平高于正常组织(P<0.05),miR-150水平低于正常组织(P<0.05);患者皮损组织中CXCL8蛋白表达率为68.75%,明显高于正常组织的37.50%(P<0.05);在患者皮损组织中CXCL8蛋白的表达水平明显高于正常组织(P<0.05);miR-150水平随着病情的加重逐渐降低(P<0.05),CXCL8蛋白水平随着病情的加重逐渐升高(P<0.05);Pearson结果显示,寻常型银屑病患者miR-150水平与CXCL8蛋白水平及PASI评分之间均呈负相关(r=-0.467、-0.475,P<0.05),CXCL8蛋白水平与PASI评分之间呈正相关(r=0.424,P<0.05)。结论寻常型银屑病患者miR-150表达降低,CXCL8表达升高,二者均与患者病情严重程度相关。Objective To investigate the relationship between the expression of CXC chemokine ligand 8(CXCL8)and miR-150 in the skin lesions of patients with psoriasis vulgaris and the severity of the disease.Methods The skin lesions of 80 patients with psoriasis vulgaris who were treated in Nanyang first people's Hospital from January 2020 to May 2021 were selected as the research objects.The normal tissues around the skin lesion were chosen as a control.The Psoriasis Area and Severity Index(PASI)was used to classify patients with psoriasis,the immunohistochemical method was used to detect the expression of CXCL8 protein,the real time fluorescence quantitative PCR method was used to detect the expression of miR-150,Western blot was used to detect the expression of CXCL8 protein,and the correlation between the expression levels of CXCL8 protein,miR-150 and the severity of the disease was further analyzed.Results The level of CXCL8 mRNA in the skin lesion of patients with psoriasis vulgaris was higher than that in normal tissues(P<0.05)and the level of miR-150 was lower than that in normal tissues(P<0.05).The expression of CXCL8 protein in the skin lesions of patients was 68.75%,which was significantly higher than 37.50%in normal tissues(P<0.05);the expression of CXCL8 protein in the skin lesions of patients was significantly higher than that in normal tissues(P<0.05);the level of miR-150 gradually decreased with the aggravation of the disease(P<0.05),and the level of CXCL8 protein gradually increased with the aggravation of the disease(P<0.05);Pearson results showed that there was a negative correlation between miR-150 level in patients with psoriasis vulgaris and CXCL8 protein level and PASI score(r=-0.467,-0.475,P<0.05),and there was a positive correlation between CXCL8 protein level and PASI score(r=0.424,P<0.05).Conclusions The expression of miR-150 decreased and CXCL8 increased in patients with psoriasis vulgaris,both of them were related to the severity of the disease.

关 键 词：寻常型银屑病 CXC趋化因子配体8 miR-150 病情严重程度 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]