瓯江彩鲤Scarb 1基因敲除自交子一代的体色相关性状观察  

Observation on body color related traits in inbred progeny of Scarb 1 knockout mutants in Oujiang color common carp

作　　者：许沁宇 刘帛屿 喻杨乐夫 王军[1] 王成辉[1] XU Qinyu;LIU Boyu;YU Yanglefu;WANG Jun;WANG Chenghui(Key Laboratory of Freshwater Aquatic Genetic Resources Certificated by the Ministry of Agriculture and Rural Affairs/National Demonstration Centre for Experimental Fisheries Science Education/Shanghai Engineering Research Center of Aquaculture,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]农业农村部淡水水产种质资源重点实验室,水产科学国家级实验教学示范中心,上海水产养殖工程技术研究中心,上海海洋大学,上海201306

出　　处：《水产科技情报》2023年第1期14-19,共6页Fisheries Science & Technology Information

基　　金：国家自然科学基金项目(32172959)。

摘　　要：为了解瓯江彩鲤基因敲除体在自交繁育过程中的体色遗传和变异情况,对野生型和敲除了Scarb 1基因的敲除型自繁F_(1)全红、粉玉体色瓯江彩鲤个体的鳞片色素细胞进行了观察比较,并测定了其体内的类胡萝卜素含量。结果显示:野生型全红瓯江彩鲤的鳞片含有黄色素细胞、红色素细胞和虹彩细胞,而敲除型全红瓯江彩鲤的鳞片上只有黄色素细胞和虹彩细胞,没有红色素细胞;野生型和敲除型粉玉瓯江彩鲤的鳞片色素细胞均为虹彩细胞,没有黄色素和红色素细胞;野生型全红瓯江彩鲤的肠道、血浆、肌肉和皮肤中的类胡萝卜素含量均显著高于敲除型全红个体(P<0.05);野生型粉玉瓯江彩鲤的肠道、肌肉和鳍条中的类胡萝卜素含量也显著高于敲除型粉玉个体(P<0.05)。研究结果表明,敲除Scarb 1基因影响了瓯江彩鲤体表红色素细胞的生成,该基因在瓯江彩鲤红、白体色调控中发挥作用。In this study,pigment cells and carotenoid contents of scales from individuals with the WR(whole red)and WW(whole white)coloration of Oujiang color common carp were observed and measured in both the gene edited mutant F_(1) and the wild fish.The results showed that the scales of the wild-type WR Oujiang color common carp contained xanthophore,erythrophore and iridescent cells,whereas the scales of the mutant type WR contained only xanthophore and iridocyste but no erythrophore.Both scale pigmented cells of wild-type and mutant type WW Oujiang color common carp were iridocyste with no xanthophore and erythrophore.Carotenoids in the gut,plasma,muscle,and skin of wild-type WR individuals were all significantly higher(P<0.05)than those of mutant type WR individuals;Carotenoid contented in the gut,muscle,and fin strips of wild-type WW were also significantly higher(P<0.05)than those of mutant type WW.The present study indicated that knock out of Scarb 1 affect the production of red pigment cells on the body surface of Oujiang color common carp,which played a role in the control of red and white coloration in the Oujiang color common carp.

关 键 词：瓯江彩鲤 基因编辑 Scarb 1基因 体色 色素细胞 类胡萝卜素 

分 类 号：S917.4[农业科学—水产科学]