HPLC法对鱼腥草中马兜铃酸A的定性分析  

Qualitative Analysis of Aristolochic Acid A in Houttuynia Cordata by HPLC

作　　者：何曼文 郭海嘉 黎芷君 许健翔 李忆东 冯丽雄 He Manwen;Guo Haijia;Li Zhijun;Xu Jianxiang;Li Yidong;Feng Lixiong(School of Applied Biology,Shenzhen Institute of Technology,Shenzhen 518116,China)

机构地区：[1]深圳技师学院应用生物学院,广东深圳518116

出　　处：《山东化工》2022年第24期111-113,共3页Shandong Chemical Industry

基　　金：深圳技师学院校科研项目(批准号:2211029)资助的课题。

摘　　要：目的:建立高效液相色谱法检测鱼腥草中马兜铃酸A的分析方法。方法先用索氏提取法对鱼腥草进行提取,再采用安捷伦Agilent C18色谱柱(250 mm×4.6 mm,5μm,美国),流动相:甲醇-3.3%冰醋酸(体积比70∶30),流速:1.0 mL/min,柱温:室温,检测波长:250 nm进行检测。结果:鱼腥草全株(湖北麻城)均未检出马兜铃酸A(检出限为5.7×10^(-5)μg)。结论:鱼腥草全株及其制剂不会引起马兜铃酸导致的安全性问题。Objective:To establish a method for the determination of aristolochic acid A in Houttuynia cordata by high performance liquid chromatography.Methods:Soxhlet extraction was used to extract Houttuynia cordata,and then an Agilent C18chromatographic column(250 mm×4.6 mm,5μm,USA)was used.Methanol-3.3%glacial acetic acid(70∶30)was used as the mobile phase,and the flow rate was 1.0 mL/min.The column temperature is room temperature,and the detection wavelength 250 nm was used for detection.Results:Aristolochic acid A(The limit of detection was 5.7×10^(-5)μg)was not detected in the whole plant of Houttuynia cordata(Macheng,Hubei).Conclusion:The whole plant of Houttuynia cordata and its preparations do not pose safety problems caused by aristolochic acid.

关 键 词：鱼腥草 马兜铃酸A HPLC法 索氏提取法 定性分析 

分 类 号：TQ461[化学工程—制药化工] R284[医药卫生—中药学]