基于斜率比法评定断奶仔猪对不同铜源的相对生物学利用率  

Evaluation of Relative Bioavailability of Copper Sources Based on Slope Ratio Method for Weanling Piglets

作　　者：晏家友[1,2] 罗宗刚 李书伟[4,5] 汤文杰[4,5] 邝声耀 YAN Jiayou;LUO Zonggang;LI Shuwei;TANG Wenjie;KUANG Shengyao(Sichuan Animal Science Academy,Chengdu 610066,China;Animal Breeding and Genetics key Laboratory of Sichuan Province,Chengdu 610066,China;College of Animal Science and technology,Southwest University,Rongchang 402460,China;Sichuan Animtech Feed Co.,Ltd.,Chengdu 610066,China;Livestock Biological Products Key Laboratory of Sichuan Province,Shuangliu 616200,China)

机构地区：[1]四川省畜牧科学研究院,成都610066 [2]动物遗传育种四川省重点实验室,成都610066 [3]西南大学动物科学技术学院,荣昌402460 [4]四川省畜科饲料有限公司,成都610066 [5]畜禽生物制品四川省重点实验室,双流616200

出　　处：《动物营养学报》2023年第1期148-156,共9页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：四川省引智成果示范推广(2022ZHYZ0004);四川省省级财政运行专项(SASA2022CZYX005)。

摘　　要：本试验旨在研究不同铜源(硫酸铜、碱式氯化铜和柠檬酸铜)和铜添加水平(20和30 mg/kg)对断奶仔猪生长性能、血清铜含量与含铜酶活性以及组织铜含量的影响,探讨断奶仔猪对不同铜源的相对生物学利用率。试验采用3×2双因子随机区组设计,选取平均体重为(8.98±0.48)kg的杜×长×大三元杂交断奶仔猪112头,随机分为7组,每组4个重复,每个重复4头猪。对照组饲喂玉米-豆粕型基础饲粮(铜含量为7.80 mg/kg),各试验组分别在基础饲粮中添加20或30 mg/kg硫酸铜、碱式氯化铜或柠檬酸铜(均以铜含量计)。试验预试期3 d,正试期28 d。结果表明:1)试验组与对照组断奶仔猪平均日增重、平均日采食量和料重比均无显著差异(P>0.05)。2)饲粮铜源和铜添加水平对断奶仔猪血清铜含量具有显著影响(P<0.05),但对血清铜蓝蛋白和铜锌超氧化物歧化酶活性均无显著影响(P>0.05)。3)饲粮铜源和铜添加水平对断奶仔猪肝脏铜含量具有显著影响(P<0.05),但对心脏、肾脏、胰脏和跖骨铜含量均无显著影响(P>0.05)。4)根据多元线性回归斜率比法计算,以血清铜含量为评价指标,碱式氯化铜和柠檬酸铜相对于硫酸铜(100%)的生物学利用率分别为110.39%和120.78%;以肝脏铜含量为评价指标,碱式氯化铜和柠檬酸铜相对于硫酸铜(100%)的生物学利用率分别为119.39%和136.09%。综上所述,本试验条件下,断奶仔猪对低铜饲粮(铜添加水平为20或30 mg/kg)中3种铜源的相对生物学利用率表现为柠檬酸铜>碱式氯化铜>硫酸铜。This experiment was conducted to study the effects of different copper(Cu)sources[copper sulfate(CuSO4),tribasic copper chloride(TBCC)and cupric citrate(CuCit)]and Cu supplemental levels(20 and30 mg/kg)on growth performance,serum Cu content and Cu-containing enzyme activities,and tissue Cu content of weanling piglets,and to determine the relative bioavailability of different Cu sources. A total of 112 Duroc×Landrace×Yorkshire crossbred weanling piglets,with the initial body weight of(8.98±0.48)kg,were randomly assigned to seven groups with four replicates per group and four piglets per replicate,according to the randomized block 3×2 two factorial design. The piglets in control group were fed a corn-soybean meal basal diet(7.8 mg/kg Cu)and the diets of experimental groups included the basal diet supplemented with 20 or30 mg/kg Cu as CuSO4,TBCC or CuCit,respectively. The piglets were raised for 28 days after 3 days adaptation. The results showed as follows:1)there were no significant differences in average daily gain,average daily feed intake and feed to gain ratio among control group and experimental groups(P>0.05). 2)Dietary Cu source and Cu supplemental level significantly affected the serum Cu content(P<0.05),but did not significantly affect the activities of ceruloplasmin(CP)and copper-zinc superoxide dismutase(Cu/Zn-SOD)in serum(P>0.05). 3)The liver Cu content was significantly affected by dietary Cu source and Cu supplemental level(P<0.05),but Cu content in heart,kidney,pancreas and metatarsal bone was not significantly affected by dietary Cu source and Cu supplemental level(P>0.05). 4)According to slope ratio method from multiple linear regression,the bioavailability values of TBCC and CuCit relative to CuSO4(100%)were 110.39% and120.78%,respectively,based on the serum Cu content as evaluating indicator. Moreover,the bioavailability values of TBCC and CuCit relative to CuSO4(100%)were 119.39% and 136.09%,respectively,based on the liver Cu content as evaluating indicator. In summary,under this experiment

关 键 词：铜源 断奶仔猪 相对生物学利用率 斜率比法 

分 类 号：S823[农业科学—畜牧学]