山羊ZBP1基因多态性及与产羔性能的关联分析  

Association Analysis Between Polymorphisms of ZBP1 Gene and Lambing Performance in Goats

作　　者：刘泽林 杨娟 许铭洙 张年[2] 熊琪[2] 刘静波[1] 陶虎[2] LIU Zelin;YANG Juan;XU Mingzhu;ZHANG Nian;XIONG Qi;LIU Jingbo;TAO Hu(School of Life Science and Engineering,Southwest University of Science and Technology,Mianyang 621010,China;Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding,Institute of Animal Husbandry and Veterinary,Hubei Academy of Agricultural Sciences,Wuhan 430064,China)

机构地区：[1]西南科技大学生命科学与工程学院,绵阳621010 [2]湖北省农业科学院畜牧兽医研究所,动物胚胎工程及分子育种湖北省重点实验室,武汉430064

出　　处：《中国畜牧兽医》2023年第1期161-173,共13页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31972995);湖北省重点研发计划项目(2021BBA086、2022BBA0049);湖北揭榜制科技项目(2020BED028)。

摘　　要：【目的】试验旨在分析山羊Z-DNA结合蛋白(Z-DNA binding protein 1,ZBP1)基因3′-端非翻译区(3′-UTR)、内含子1和外显子7区域中单核苷酸多态性(single nucleotide polymorphism,SNP)位点与产羔性能之间的关联性,为高繁殖力山羊分子育种提供新的遗传标记。【方法】选取麻城黑山羊、黑头羊和波尔山羊作为研究样本,采集血样提取DNA,根据转录组数据所选SNP位点在山羊ZBP1基因序列中的位置信息设计引物,利用多重单碱基延伸SNP分型技术(SNaPshot)分析所选SNP位点的遗传多样性,采用一般线性模型(GLM)对ZBP1基因SNP位点与3个品种山羊的产羔性能进行关联分析。【结果】山羊ZBP1基因中存在12个潜在的SNPs位点,其中7个SNPs位点(g.9734 A>G、g.9772 T>C、g.352 C>T、g.955 C>T、g.1880 G>A、g.2566 T>C和g.7919 G>A)具有多态性,除g.9734 A>G在麻城黑山羊群体中仅有AA和GA 2种基因型外,其余6个SNPs位点在麻城黑山羊、波尔山羊和黑头羊群体中均表现为CC(AA)、CT(GA)和TT(GG)3种基因型。波尔山羊群体中g.9734 A>G、g.9772 T>C位点表现为低度多态(PIC<0.25);麻城黑山羊群体中g.9734 G>A和g.7919 G>A位点表现为低度多态(PIC<0.25),g.9772 T>C、g.352 C>T、g.955 C>T、g.1880 G>A和g.2566 T>C位点表现为中度多态(0.25G、g.352 C>T和g.7919 G>A位点表现为低度多态(PIC<0.25)。卡方适应性检测结果表明,g.9734 A>G、g.9772 T>C和g.352 C>T位点在3个山羊群体中均处于Hardy-Weinberg平衡状态(P>0.05),波尔山羊及麻城黑山羊群体中g.2566 T>C位点处于Hardy-Weinberg平衡状态(P>0.05)。关联分析结果显示,g.9772 T>C位点与波尔山羊头胎产羔数显著关联(P<0.05),g.352 C>T和g.7919 G>A位点与波尔山羊总体产羔数均显著或极显著关联(P<0.05;P<0.01);g.352 C>T位点与黑头羊总体产羔数极显著关联(P<0.01);g.9772 T>C位点与麻城黑山羊头胎产羔数显著关联,g.9734 A>G和g.9772 T>C位点与麻城黑山羊总体产羔数均显著关联(【Objective】 The purpose of this experiment was to study the association between single nucleotide polymorphism(SNP) in 3′-UTR,intron 1 and exon 7 of Z-DNA binding protein 1(ZBP1) gene and lambing performance in goats, and provide new genetic markers for molecular breeding of high fertility goats.【Method】 Macheng Black goats, Black headed goats and Boer goats were selected as research samples, the blood samples were collected and DNA was extracted.Primers were designed according to the location information of SNP selected from transcriptome data in ZBP1 gene sequence of goats.Multiple single base extension SNP typing technique(SNaPshot) was used to analyze the genetic diversity of ZBP1 gene SNP.General linear model(GLM) was used to analyze the relationship between SNP of ZBP1 gene and lambing performance of 3 goat breeds.【Result】 There were 12 potential SNPs of ZBP1 gene in goats, of which 7 SNPs(g.9734 A>G,g.9772 T>C,g.352 C>T,g.955 C>T,g.1880 G>A,g.2566 T>C and g.7919 G>A) were polymorphic.There were 2 genotypes of AA and GA at g.9734 A>G in Macheng Black goats population, the other 6 SNPs showed 3 genotypes including CC(AA),CT(GA) and TT(GG) in Macheng Black goats, Boer goats and Black headed goats.g.9734 A>G and g.9772 T>C showed low polymorphism in Boer goats population(PIC<0.25),g.9734 G>A and g.7919 G>A showed low polymorphism in Macheng Black goats population(PIC<0.25),while g.9772 T>C,g.352 C>T,g.955 C>T,g.1880 G>A and g.2566 T>C showed moderate polymorphism in Black headed goats population(0.25G,g.352 C>T and g.7919 G>A showed low polymorphism in Black headed goats(PIC<0.25).The chi-square fitness test results show that g.9734 A>G,g.9772 T>C and g.352 C>T were in Hardy-Weinberg equilibrium in 3 goat populations(P>0.05),g.2566 T>C in Boer goats and Macheng Black goats population was in Hardy-Weinberg equilibrium(P>0.05).Correlation analysis results showed that g.9772 T>C was significantly associated with the first litter sizes in Boer goats(P<0.05),g.352 C>T and g.7919 G>A were significantly

关 键 词：山羊 ZBP1基因 多态性 产羔性能 关联分析 

分 类 号：S813.3[农业科学—畜牧学]