基于形变距离和受压质量混合模式测定蓝莓果实硬度的方法建立和优化  

Foundation and optimization of protocol for blueberry fruit firmness measurement under mix mode of deflection distance and pressure weight

作　　者：沈朱俐 顾莉莉 李晓谊 李永强[1,2] 宗宇 徐丽珊[1,2] 郭卫东 SHEN Zhuli;GU Lili;LI Xiaoyi;LI Yongqiang;ZONG Yu;XU Lishan;GUO Weidong(College of Chemistry and Life Sciences,Zhejiang Normal University,Jinhua 321004,Zhejiang,China;Zhejiang Provincial Key Laboratory of Biotechnology on Specialty Economic Plants,Jinhua 321004,Zhejiang,China)

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004 [2]浙江省特色经济植物生物技术重点实验室,浙江金华321004

出　　处：《果树学报》2023年第1期169-179,共11页Journal of Fruit Science

基　　金：浙江省农业新品种选育重大科技专项(2021C02066-9);浙江省科技厅重点研发计划项目(2018C02007);浙江省大学生科技创新活动计划暨新苗人才计划(2021R404079)。

摘　　要：【目的】探究不同发育时期蓝莓果实在不同测量模式下的硬度差异,建立和优化蓝莓果实硬度测定的方法,分析蓝莓果实硬度育种的趋势。【方法】以蓝莓品种奥尼尔的果实为试材,利用不同形变阈值和压力阈值模式对发育期的果实硬度进行了测定分析,基于优化的方法测定36个蓝莓品种成熟果实的硬度。【结果】当蓝莓果实取样量超过45个时可以获得稳定的果实硬度测量值,取样量为60~75个时测定的果实硬度更适合作为品种的特征值。在形变测定模式下,果实形变阈值为1.0 mm时更适用于发育前期的S3和S4时期果实,形变阈值为1.5和2.0 mm更适用于硬度逐渐下降的S5~S8时期果实;在压力测定模式下,400 g压力阈值更适用于测定S3到S8时期的果实硬度。蓝莓果实硬度随品种选育时间逐渐增大,高丛蓝莓近期育成品种的果实硬度一般大于老品种,该特征在南高丛蓝莓中表现更为突出。【结论】在蓝莓果实硬度测定中采用400 g压力阈值与2.0 mm形变阈值混合模式,或者1.0和2.0 mm结合的形变阈值模式测定发育期蓝莓果实的硬度。南高丛蓝莓明星和密斯蒂、北高丛蓝莓德雷伯、兔眼蓝莓沃农和梯芙蓝可以作为硬肉型蓝莓品种选育的优良亲本。【Objective】Fruit firmness of blueberry(Vaccinium spp.)at different developmental stages were measured under diverse force and deflection testing modes.We aimed to found and optimize the protocol for blueberry firmness determination.In addition,we also wanted to summarize the breeding tendency of blueberry for high fruit firmness.【Methods】Assuming that the sample size was an important element for study design and validation of fruit firmness,we assessed how the mean firmness varied with different sample sizes using the ripe fruits of four blueberry cultivars Emerald,Jewel,O’Neal and Sharpblue.The optimal sampling amount for blueberry fruit firmness measurement was statistically analyzed.The fruit firmness was analyzed at six developmental(from S3 to S8)stages using different deflection thresholds and force threshold modes using the fruits of blueberry cultivar O’Neal.Optimized measurements was constructed for firmness detection of blueberry fruits.Using the complementary methods,we determined the fruit firmness 36 blueberry cultivars.The breeding tendency of fruit firmness of different blueberry groups was proposed by combing the correlation coefficient between the fruit firmness and the release year of blueberry cultivar,and by considering the cultivar phylogeny.【Results】The sample sizes showed pivotal an effect on variance in the mean firmness values of blueberry genotypes.Taking discrete degree of fruit firmness,the values out of the confidence intervals,and the coefficient of variation of fruit firmness under different sample sizes into account,we found stable and reliable observation value of fruit firmness could be obtained when the sampling amount of blueberry fruit exceeded 45 berries.However,a sampling amount ranging from 60 to 75 was advised to stand for the characteristic fruit firmness of the blueberry cultivar.The formation process of blueberry fruit firmness could be divided into two stages,with the first stage from S3 to S4 and the second stage from S5 to S8.Fruit firmness at stages

关 键 词：蓝莓 果实硬度 形变 受力 

分 类 号：S663.2[农业科学—果树学]