三卤甲烷对秀丽隐杆线虫生殖细胞及子代数的影响  

Effect of trihalomethanes on germ cells and brood size of Caenorhabditis elegans

作　　者：吴佳洁 周童 曹振霄 凤玉 许安[1,2] 杜华 Wu Jiajie;Zhou Tong;Cao Zhenxiao;Feng Yu;Xu An;Du Hua(High Magnetic Field Key Laboratory,Hefei Institutes of Physical Science,Chinese Academy of Sciences,Hefei 230031;Science Island Branch of Graduate School,University of Science and Technology of China,Hefei 230026)

机构地区：[1]中国科学院合肥物质科学强磁场科学中心,合肥230031 [2]中国科学技术大学研究生院科学岛分院,合肥230026

出　　处：《安徽医科大学学报》2023年第1期5-9,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:22006149)。

摘　　要：目的探讨3种三卤甲烷类饮用水消毒副产物对秀丽隐杆线虫生殖细胞发育与繁殖能力的影响。方法以3种三卤甲烷(三溴甲烷、三氯甲烷、三碘甲烷)为研究对象,在秀丽隐杆线虫模型中检测不同浓度三卤甲烷暴露后生殖腺细胞凋亡和卵母细胞数,及最终产生的子代数目。结果生殖腺细胞凋亡结果显示,50μmol/L处理组中三氯甲烷和三碘甲烷的暴露造成线虫生殖腺凋亡水平升高,且存在一定剂量依赖效应。卵母细胞数结果显示,在50μmol/L浓度暴露下,3种THMs的暴露均显著降低了线虫体内的卵母细胞数。子代数结果显示,仅有50μmol/L处理组的三碘甲烷暴露会显著降低线虫的子代数。结论THMs会造成秀丽隐杆线虫的生殖毒性,且毒性与其携带的卤族元素有关。Objective To investigate the effects of 3 types of trihalomethanes(THMs),the by-products in treatment of drinking water disinfection,on the development and reproductive capacity of germ cells ofCaenorhabditis elegans.Methods Tribromomethane(TBM),trichloromethane(TCM)and triiodomethane(TIM),3 types of THMs were selected and wild typeCaenorhabditis eleganswas used as a model to detect germ cell apoptosis,oocyte number and the final brood size after exposure to different concentrations of THMs.Results The results of germ cell apoptosis showed that the exposure to 50μmol/L TCM and TIM caused elevated levels of germ cell apoptosis in a dose-dependent manner.Oocyte counting results showed that exposure to 50μmol/L all three THMs significantly reduced the number of oocytes.The brood size results showed that only 50μmol/L TIM exposure significantly decreased the brood size of nematodes.Conclusion THMs showed negative impacts on the reproductive system of Caenorhabditis elegans,and the toxicity is related to the halogenated elements of THMs.

关 键 词：三卤甲烷 秀丽隐杆线虫 生殖腺细胞凋亡 卵母细胞数 子代数 

分 类 号：R123.1[医药卫生—环境卫生学]