FAK-PI3K/AKT信号通路在新生大鼠颅盖骨生长发育过程中作用机制的研究  

Study on the mechanism of FAK-PI3K/AKT signal pathway in the growth and development of neonatal rat cranial bone

作　　者：张司玺 张晓璐 王雷 马玉博 邵国 张春阳[2] Zhang Sixi;Zhang Xiaolu;Wang Lei;Ma Yubo;Shao Guo;Zhang Chunyang(Baotou Medical College of Inner Mongolia University of Science and Technology,Baotou 014010;Dept of Neurosurgery,The First Affiliated Hospital of Baotou Medical College of Inner Mongolia University of Science and Technology,Baotou 014010;Dept of Endocrinology,The Third Hospital of Qinhuangdao,Qinhuangdao 066000;Translational Medicine Center,Third People's Hospital of Longgang District,Shenzhen 518100)

机构地区：[1]内蒙古科技大学包头医学院,包头014010 [2]内蒙古科技大学包头医学院第一附属医院神经外科,包头014010 [3]秦皇岛市第三医院内分泌科,秦皇岛066000 [4]龙岗区第三人民医院转化医学中心,深圳518100

出　　处：《安徽医科大学学报》2023年第1期109-115,共7页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:82160250,81960238);内蒙古自然科学基金(编号:2019BS08007,2021SHZR1205)。

摘　　要：目的通过对不同时期的SD大鼠颅盖骨进行检测,探究大鼠颅盖骨的生长特点。方法选取同窝1、4、7、10、12周SD大鼠颅盖骨(每周3只),使用Real-time PCR和Western blot技术检测颅盖骨中局部粘着斑激酶(FAK)-磷脂酰肌醇3-激酶/蛋白激酶B(PI3K/AKT)信号通路的表达情况,并通过相关性分析FAK-PI3K/AKT在颅盖骨生长发育过程中的作用情况。结果大鼠脑容积的增长与颅盖骨厚度的变化是同步增长;FAK的表达变化与大鼠各经线的变化呈正相关;FAK的表达变化与PI3K/AKT的表达变化呈正相关。结论FAK的表达变化与大鼠头颅的生长发育规律具有相关性,FAK通过激活PI3K/AKT信号通路在颅盖骨中发挥作用,FAK可能作为颅骨快速生长发育期的标志物,为临床上的颅骨缺损修复治疗中时机的选择提供基础理论依据。Objective To explore the growth characteristics of rat calvaria by detecting the calvaria of SD rats in different periods.Methods The calvaria of SD rats at 1,4,7,10,and 12 weeks from the same littermate were selected(3 rats per week).Real-time PCR and Western blot techniques were used to detect the expression of focal adhesion kinase(FAK)-phosphatidylinositol 3 kinase/protein kinase B(PI3K/AKT)signal pathway in the calvaria,and the role of FAK-PI3K/AKT in the growth and development of the calvaria was analyzed by correlation.Results The increase of brain volume and the thickness of calvaria increased synchronously,the expression of FAK was positively correlated with the changes of meridians,and the expression of FAK was positively correlated with the expression of PI3K/AKT.Conclusion The expression of FAK is related to the growth and development of rat skull.FAK plays a role in calvaria by activating PI3K/AKT signal pathway.FAK may be used as a marker of rapid skull growth and development,which provides a basic theoretical basis for the timing of clinical skull defect repair and treatment.

关 键 词：颅盖骨 FAK PI3K/AKT 生长发育 

分 类 号：R319[医药卫生—基础医学]