阿魏酸钠经RhoA和Rho-kinase信号通路抑制小鼠肝纤维化的机制研究  

Mechanism of Sodium Ferulate Inhibiting Liver Fibrosis in Mice Via RhoA and Rho-kinase Signaling Pathway

作　　者：赵蔚林 李君 ZHAO Weilin;LI Jun(Xiangtan Medicine and Health Vocational College,Xiangtan City,Hu’nan Province 411102)

机构地区：[1]湘潭医卫职业技术学院,湖南省湘潭市411102

出　　处：《医学理论与实践》2023年第3期361-364,383,共5页The Journal of Medical Theory and Practice

基　　金：湘潭市科技局2021年科技研究项目(CG-ZDJH202135)。

摘　　要：目的:探讨阿魏酸钠对于四氯化碳诱导的小鼠肝纤维化的抑制机制。方法:将CL级昆明属小鼠随机分为正常对照组、CCl4-花生油模型组(模型组)、阿魏酸钠给药组(给药组),每组20只。模型组小鼠和给药组小鼠分别给予CCl4-花生油(1∶1,V/V)1ml/kg灌胃,正常对照组小鼠给予同等剂量生理盐水,三组均为1次/d,连续8周;从第9周开始,给药组小鼠给予阿魏酸钠20mg/kg灌胃,1次/d,连续给药4周。12周后,检测各组小鼠肝功能水平、肝影像学指标、病理变化及肝脏中RhoA、Rho-kinase的总体情况。结果:通过正常对照组、模型组及给药组的小鼠肝功能和肝纤维化指标等指标对比,发现阿魏酸钠能显著抑制小鼠肝纤维化程度。结论:阿魏酸钠可能通过调节RhoA和Rho-kinase信号通路抑制小鼠肝纤维化。Objective:To investigate the inhibitory mechanism of sodium ferulate on carbon tetrachloride induced liver fibrosis in mice.Methods:Male CL mice were randomly divided into three groups:normal control group,CCl4peanut oil model group(model group)and sodium ferulate administration group(administration group),with 20 each.In addition to the normal control group,the remaining mice were injected with 1ml/kg of CCl4peanut oil(1∶1,V/V)by gavage to establish the mouse liver fibrosis model once a day for 8 weeks.At the same time,mice in the normal control group were injected into the abdominal cavity with the same dose of normal saline for 8 weeks.After 8 weeks,the mice in the administration group were injected with sodium ferulate into the abdominal cavity once a day for 4 weeks at a dose of 20mg/kg.The mice were weighed(fasting),anesthetized and sampled with pentobarbital sodium,and the main clinical diagnostic indexes of liver fibrosis:liver function level,liver imaging indexes,pathological detection and liver fibrosis indexes,as well as the overall situation of RhoA and Rho-kinase in the liver of mice in each group.Results:Comparing the indexes of liver function and liver fibrosis in normal control group,model group and administration group,it was found that sodium ferulate could significantly inhibit the degree of liver fibrosis in mice.Conclusion:Sodium ferulate can inhibit liver fibrosis by regulating RhoA and Rho-kinase signaling pathways.

关 键 词：阿魏酸钠 肝纤维化 RhoA和Rho-kinase信号通路 

分 类 号：R33[医药卫生—人体生理学]