Fast fluorescence lifetime imaging techniques:A review on challenge and development  

作　　者：Xiongbo Liu Danying Lin Wolfgang Becker Jingjing Niu Bin Yu Liwei Liu Junle Qu 

机构地区：[1]Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen University Shenzhen 518060,P.R.China [2]Becker&Hickl GmbH Nunsdorfer Ring 7-9,Berlin 12277,Germany

出　　处：《Journal of Innovative Optical Health Sciences》2019年第5期3-29,共27页创新光学健康科学杂志（英文）

基　　金：support from the National Key R&D Program of China(2017YFA0700500);National Natural Science Foundation of China(61775144/61525503/61620106016/61835009/81727804);(Key)Project of Department of Education of Guangdong Province(2015KGJHZ002/2016KCXTD007);Guangdong Natural Science Foundation(2014A030312008,2017A030310132,2018A030313362);Shenzhen Basic Research Project(JCYJ20170818144012025/JCYJ20170818141701667/JCYJ20170412105003520/JCYJ20150930104948169).

摘　　要：Fluorescence lifetime imaging microscopy(FLIM)is increasingly used in biomedicine,material science,chemistry,and other related research fields,because of its advantages of high specificity and sensitivity in monitoring cellular microenvironments,studying interaction between proteins,metabolic state,screening drugs and analyzing their efficacy,characterizing novel materials,and diagnosing early cancers.Understandably,there is a large interest in obtaining FLIM data within an acquisition time as short as possible.Consequently,there is currently a technology that advances towards faster and faster FLIM recording.However,the maximum speed of a recording technique is only part of the problerm.The acquisition time of a FLIM image is a complex function of many factors.These include the photon rate that can be obtained from the sample,the amount of information a technique extracts from the decay functions,the fficiency at which it determines fluorescence decay parameters from the recorded photons,the demands for the accuracy of these parameters,the number of pixels,and the lateral and axial resolutions that are obtained in biological materials.Starting from a discussion of the parameters which determine the acquisition time,this review will describe existing and emerging FLIM techniques and data analysis algo-rithms,and analyze their performance and recording speed in biological and biomedical applications.

关 键 词：Fluorescence lifetime imaging microscopy(FLIM) acquisitin time imaging speed dead time photon fficiency time domain frequency domain scanning wide-field imaging time-correlated single photon counting(TCSPC) gated detection gated image intensifer modulated inage intensifier SPAD array detector 

分 类 号：O57[理学—粒子物理与原子核物理]